User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/24: Difference between revisions
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* | * Transduction Day 2: change media | ||
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''' | '''Transduction Day 2'''<br> | ||
* Check GFP controls | |||
** Plate 1: U2OS, ~90% GFP expression, at least ~two doubling | |||
** Plate 2: SK-N-SH, ~90% expression, slow cell growth | |||
** Plate 4: K562, <font color="red">no GFP expression</font> | |||
* Remove media | |||
* Add 2 ml/well fresh media with 1% Pen/strep | |||
* Grow cells at 37°C (overnight) | |||
Revision as of 06:56, 28 September 2014
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09/24/14
Transduction Day 2
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