User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/30: Difference between revisions
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** Added 400 μL virus (thawed/ spun/ pipetted to remix) to each cell sample. Swirled to mix. (rinsed pipette tip before discarding) | ** Added 400 μL virus (thawed/ spun/ pipetted to remix) to each cell sample. Swirled to mix. (rinsed pipette tip before discarding) | ||
***Note: had some left-over virus; marked these with black dot on caps, placed back into the -80°C box | ***Note: had some left-over virus; marked these with black dot on caps, placed back into the -80°C box | ||
** Spun at 2250 | ** Spun at 2250 rpm (1178 rcf) for 25 min. | ||
** Pipetted u/d to resuspend, transferred cells to 6 mL plate | ** Pipetted u/d to resuspend, transferred cells to 6 mL plate | ||
** Grew at 37°C. Will check GFP expression tomorrow. | ** Grew at 37°C. Will check GFP expression tomorrow. |
Revision as of 11:40, 8 October 2014
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09/30/14
K562 transduction (trial 2)
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