User:Karmella Haynes/Notebook/PcTF Genomics/2014/10/18: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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* Luc14 cells - Spinoculation for Jonah's samples (Biodesign) | * Luc14 cells - Spinoculation for Jonah's samples (Biodesign) | ||
* K562 - Spinoculation (Haynes lab) | * K562 - Spinoculation (Haynes lab) | ||
* Passages - SK-N-SH, Gal4EED/Luc +dox | * Passages - SK-N-SH, U2OS, Gal4EED/Luc +dox | ||
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* Take half of the transfected sample (2 mL), spin at 2000 rpm for 10 min, replate | * Take half of the transfected sample (2 mL), spin at 2000 rpm for 10 min, replate | ||
* Final volumes for samples was 2mL. Did not bring up to 4 mL | * Final volumes for samples was 2mL. Did not bring up to 4 mL | ||
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'''Passages - SK-N-SH, U2OS, Gal4EED/Luc +dox''' | |||
SK-N-SH | |||
* T-75: 1:5 | |||
* 6-well plate: 0.5 mL per well (will switch to p/s-free before spinoculation transfection) | |||
U2OS | |||
* T-75: 1:5 | |||
* T-75: 1:10 | |||
Gal4EED/Luc | |||
* T-75: 2x 1:5 + 1μg/mL dox | |||
* 2x 6-well: 0.5 mL per well (will switch to p/s-free before spinoculation transfection) | |||
Latest revision as of 00:26, 27 September 2017
Pc-TF Genomics | Main project page Previous entry Next entry |
10/18/14
Luc14 cells - Spinoculation for Jonah's samples (Biodesign) Transfections set up by Jonah on Friday (yesterday). Note: cells were in p/s+ medium (complete)
Conclusions:
K562 - Spinoculation (Haynes lab)
Passages - SK-N-SH, U2OS, Gal4EED/Luc +dox SK-N-SH
U2OS
Gal4EED/Luc
|