User:Karmella Haynes/Notebook/PcTF Genomics/2014/10/18

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10/18/14

  • Luc14 cells - Spinoculation for Jonah's samples (Biodesign)
  • K562 - Spinoculation (Haynes lab)


K562 - Spinoculation (Haynes lab)

  • Try spin technique on K562 cells using 15 mL conicals
  • Lipo LTX transfection (standard protocol)
  • Take half of the transfected sample (2 mL), spin at 2000 rpm for 10 min, replate
  • Final volumes for samples was 2mL. Did not bring up to 4 mL
Reagent Volume
reagent 1 # μL
reagent 2 #
reagent 3 #
reagent 4 #
dH2O #
  # μL

--> Reaction conditions



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