User:Karmella Haynes/Notebook/PcTF Genomics/2014/11/15: Difference between revisions
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# KAH126/MV2 | # KAH126/MV2 | ||
# | # mock | ||
# no transfection | # no transfection | ||
* Grow overnight, look for RFP next day | * Grow overnight, look for RFP next day | ||
* Next day: harvest transfected cells and use 1/2 (5.0E5) for 2 different plates | * Next day: harvest transfected cells and use 1/2 (5.0E5) for 2 different plates | ||
** 24-well plate - ~2. | ** 24-well plate - ~2.1E4 cells/well | ||
** 96-well plate - | ** 96-well plate - ~5.2E4 cells/well | ||
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Revision as of 13:08, 15 November 2014
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11/15/14
Transfection: KAH126/MV2 into U2OS
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