User:Karmella Haynes/Notebook/PcTF Genomics/2014/11/16: Difference between revisions

Latest revision as of 00:31, 27 September 2017

Pc-TF Genomics

Transfection: Re-plate transfected cells

KAH126/MV2 cells in well 1 were ~50% - 60% RFP+ (very high!)
- Could be due to low passage number (these were passage-1 cells)
Re-plated cells into two plates (U2OS complete growth medium):
- 96-well: 0.2 mL per well (~5.2 E3 cells/well)
- 24-well: 2.0 mL per well (~21.0 E3 cells/well)
Tomorrow (Monday), check wells for RFP signal; if confluent re-plate high RFP samples (or wait until Tuesday)

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