User:Karmella Haynes/Notebook/PcTF Genomics/2015/03/20: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* PAGE - glucagon & insulin (for Gbox demo next week) | * PAGE - glucagon & insulin (for Gbox demo next week) | ||
---- | ---- | ||
'''PAGE - glucagon & insulin'''<br> | '''PAGE - glucagon & insulin'''<br> | ||
> Sample prep | > From David Barclay's pancreas cell project | ||
* Glucagon - | |||
* | |||
> Gel lanes | '''Sample prep''' | ||
# | * Loading vol. 20 uL = 5.0 μL 4x Novagen sample buffer (blue) + 1.0 μL 1M DTT + 14 uL protein | ||
* Dilutions - make enough for at least two lanes plus pipetting error (x3) | |||
{| class="wikitable" border="0" cellspacing="3" | |||
|- | |||
| Protein || Final mass || Stock μL (x3) || dH<sub>2</sub>O μL (x3) | |||
|- | |||
| Glucagon || 10.0 μg || 10.0 (30.0) || 4.0 (12.0) | |||
|- | |||
| Glucagon || 1.0 μg || 1.0 (3.0) || 13.0 (39.0) | |||
|- | |||
| Glucagon || 0.1 μg || 0.1 (0.3) || 13.9 (41.7) | |||
|- | |||
| Insulin* || 10.0 μg || 10.0 (30.0) || 4.0 (12.0) | |||
|- | |||
| Insulin* || 1.0 μg || 1.0 (3.0) || 13.0 (39.0) | |||
|- | |||
| Insulin* || 0.1 μg || 0.1 (0.3) || 13.9 (41.7) | |||
|} | |||
*For insulin, start with a 1/25 dilution of the 25 mg/mL stock | |||
* 4x sample buffer batch (x15) = 75.0 4x sample buffer + 15.0 1M DTT | |||
> Gel lanes (for both gels) | |||
# Page Ruler plus (15 uL) | |||
# Glucagon 10.0 μg | |||
# Glucagon 1.0 μg | |||
# Glucagon 0.1 μg | |||
# Insulin 10.0 μg | |||
# Insulin 1.0 μg | |||
# Insulin 0.1 μg | |||
# Page Ruler plus (15 uL) | |||
# Glucagon 10.0 μg | |||
# Glucagon 1.0 μg | |||
# Insulin 10.0 μg | |||
# Insulin 1.0 μg | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> | {| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table --> |
Latest revision as of 00:50, 27 September 2017
Pc-TF Genomics | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||
03/20/15
PAGE - glucagon & insulin
--> Reaction conditions
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