User:Karmella Haynes/Notebook/PcTF Genomics/2015/06/25

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mm/dd/yy

  • Cas-tone - cell transfections

Cas-tone - transfections

  • Final H3#_MV11 builds verified on 6/24/15
  • Co-transfect H3#_MV11 with pSP-guide RNA plasmid (built by Ben Nyer)
  • Use Luc14 cells (from Rene)
  1. H340_MV11 + pSP###
  2. H340_MV11 + pSP###
  3. H340_MV11 + pSP###
  4. H340_MV11 + pSP###
  5. H340_MV11 + pSP###
  6. pSP### (negative)
Well H3#_MV11 (1.0 μg) pSP# (1.0 μg) PLUS rgt. Lipo LTX
1 # μL # μL μL
2 #
3 #
4 #
5 #
6 #

--> Reaction conditions



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