User:Karmella Haynes/Notebook/PcTF Genomics/2015/08/12: Difference between revisions

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'''SDS-PAGE'''
'''SDS-PAGE'''
* Protocol: http://openwetware.org/wiki/Haynes:PAGE
* Protocol: http://openwetware.org/wiki/Haynes:PAGE
* Gel:
* Gel: Nupage 4-12% Bis-Tris
* Buffer:
* Buffer: NuPAGE® MOPS SDS
* Chamber: Invitrogen XCell SureLock
* Chamber: Invitrogen XCell SureLock



Revision as of 15:58, 12 August 2015

Pc-TF Genomics <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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08/12/15

  • Castone Western - gel and transfers

Castone Western - gel and transfers

  1. Luc14: H340_MV11 + g034_pSPgRNA
  2. Luc14: H340_MV11 (no gRNA)
  3. Luc14: H360_MV11 + g034_pSPgRNA
  4. Luc14: H360_MV11 (no gRNA)
  5. Luc14: H380_MV11 + g034_pSPgRNA
  6. Luc14: H380_MV11 (no gRNA)
  7. Gal4EED-luc: H340_MV11 + g034_pSPgRNA
  8. Gal4EED-luc: H340_MV11 (no gRNA)
  9. Gal4EED-luc: H360_MV11 + g034_pSPgRNA
  10. Gal4EED-luc: H360_MV11 (no gRNA)
  11. Gal4EED-luc: H380_MV11 + g034_pSPgRNA
  12. Gal4EED-luc: H380_MV11 (no gRNA)
  • Run two gels (1-6 and 7-12)


SDS-PAGE