User:Karmella Haynes/Notebook/Polycomb project/2010/11/15
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11/15/10
Western blot: ChIP optimization
> Loading: 25 uL total; IP samples ready to load; Input = 22.5 protein + 7.5 4x dye(+200 mM DTT)
> Block: 5% milk/PBST, RT/ > 1 hr. > Primary staining: 5% BSA/PBST, 4°C/o.n.
> Predicted sizes (using http://www.expasy.ch/tools/pi_tool.html for KAH proteins)
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