User:Kate Thodey: Difference between revisions

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==Research interests==
==Research interests==
Many drugs are still produced the traditional way, by extraction from medicinal plants. In the Smolke laboratory we are working to take a drug biosynthetic pathway from a medicinal crop – the opium poppy – and engineer the production of the same therapeutic molecules in an industrial microbe, yeast.
Many drugs are still produced the traditional way; by extraction from medicinal plants. In many cases the alternative chemical syntheses of such compounds are low-yielding due to the complex nature of these biological molecules. In the Smolke laboratory we are interested in using new synthetic biology approaches to biosynthesize a class of drug molecules which are currently produced by extraction from opium poppies, namely the benzylisoquinoline alkaloids which include the powerful analgesics codeine and morphine. As a postdoc working together with members of the laboratory, my research focuses on metabolically engineering yeast to produce opioids and other high-value alkaloids.
 
As a Postdoc working together with members of the Metabolic Engineering team, my research focuses broadly on metabolically-engineering yeast to produce opiate alkaloids such as morphine and codeine. In order to maximize the number and quantity of morphinan alkaloids we can produce by microbial fermentation, my approach is to focus on the spatial engineering of this pathway in the host yeast cell. Spatial engineering is particularly useful in this instance because in the natural system, the opium poppy, the biosynthesis of opiates takes place across several cell types and subcellular structures. It could therefore be expected that efforts to engineer this pathway within single-celled yeast would benefit from making use of the available cellular compartments. Yeast organelles could provide microenvironments that are conducive to opiate alkaloid biosynthesis due to optimal pH or the availability of cofactors; they could compartmentalize toxic metabolites or ensure a local concentration of reactive intermediates; and they may be used to support the reconstitution of enzyme complexes which form naturally in the opium poppy. In the future, we envisage that the development of yeast synthetic organelles could support further spatial engineering efforts without taxing the functioning of native organelles.


==Publications==
==Publications==
#Siddiqui MS, Thodey K, Trenchard I, Smolke CD. Advancing secondary metabolite  
#Siddiqui MS, Thodey K, Trenchard I, Smolke CD. Advancing secondary metabolite biosynthesis in yeast with synthetic biology tools. FEMS Yeast Res. 2012 Mar;12(2):144-70. doi: 10.1111/j.1567-1364.2011.00774.x. Epub 2012 Jan 11. PubMed PMID: 22136110.
biosynthesis in yeast with synthetic biology tools. FEMS Yeast Res. 2012
#Michener JK, Thodey K, Liang JC, Smolke CD. Applications of genetically-encoded biosensors for the construction and control of biosynthetic pathways. Metab Eng. 2011 Sep 18. [Epub ahead of print] PubMed PMID: 21946159; PubMed Central PMCID: PMC3256257.
Mar;12(2):144-70. doi: 10.1111/j.1567-1364.2011.00774.x. Epub 2012 Jan 11. PubMed
# Thodey K, Smolke CD. Cell biology. Bringing it together with RNA. Science.2011 Jul 22;333(6041):412-3. PubMed PMID: 21778388.
PMID: 22136110.
#Michener JK, Thodey K, Liang JC, Smolke CD. Applications of
genetically-encoded biosensors for the construction and control of biosynthetic
pathways. Metab Eng. 2011 Sep 18. [Epub ahead of print] PubMed PMID: 21946159;
PubMed Central PMCID: PMC3256257.
#Thodey K, Smolke CD. Cell biology. Bringing it together with RNA. Science.
2011 Jul 22;333(6041):412-3. PubMed PMID: 21778388.
# Schaffer RJ, Friel EN, Souleyre EJ, Bolitho K, Thodey K, Ledger S, et al. A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway. Plant Physiol. 2007;144(4):1899-912. PMCID: 1949883.
# Schaffer RJ, Friel EN, Souleyre EJ, Bolitho K, Thodey K, Ledger S, et al. A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway. Plant Physiol. 2007;144(4):1899-912. PMCID: 1949883.
# Janssen BJ, Thodey K, Schaffer RJ, Alba R, Balakrishnan L, Bishop R, et al. Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biol. 2008;8:16. PMCID: 2287172.
# Janssen BJ, Thodey K, Schaffer RJ, Alba R, Balakrishnan L, Bishop R, et al. Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biol. 2008;8:16. PMCID: 2287172.
# Walton EF, Wu RM, Richardson AC, Davy M, Hellens RP, Thodey K, et al. A rapid transcriptional activation is induced by the dormancy-breaking chemical hydrogen cyanamide in kiwifruit (Actinidia deliciosa) buds. J Exp Bot. 2009;60(13):3835-48. PMCID: 2736901.
# Walton EF, Wu RM, Richardson AC, Davy M, Hellens RP, Thodey K, et al. A rapid transcriptional activation is induced by the dormancy-breaking chemical hydrogen cyanamide in kiwifruit (Actinidia deliciosa) buds. J Exp Bot. 2009;60(13):3835-48. PMCID: 2736901.

Revision as of 07:32, 13 August 2012

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Contact Info

Kate Thodey
Smolke Laboratory
Stanford University
CA 94305

Education

  • 2005-2009, PhD, John Innes Centre, Norwich, United Kingdom
  • 2000-2003, BSc(Hons), University of Auckland, Auckland, New Zealand

Research interests

Many drugs are still produced the traditional way; by extraction from medicinal plants. In many cases the alternative chemical syntheses of such compounds are low-yielding due to the complex nature of these biological molecules. In the Smolke laboratory we are interested in using new synthetic biology approaches to biosynthesize a class of drug molecules which are currently produced by extraction from opium poppies, namely the benzylisoquinoline alkaloids which include the powerful analgesics codeine and morphine. As a postdoc working together with members of the laboratory, my research focuses on metabolically engineering yeast to produce opioids and other high-value alkaloids.

Publications

  1. Siddiqui MS, Thodey K, Trenchard I, Smolke CD. Advancing secondary metabolite biosynthesis in yeast with synthetic biology tools. FEMS Yeast Res. 2012 Mar;12(2):144-70. doi: 10.1111/j.1567-1364.2011.00774.x. Epub 2012 Jan 11. PubMed PMID: 22136110.
  2. Michener JK, Thodey K, Liang JC, Smolke CD. Applications of genetically-encoded biosensors for the construction and control of biosynthetic pathways. Metab Eng. 2011 Sep 18. [Epub ahead of print] PubMed PMID: 21946159; PubMed Central PMCID: PMC3256257.
  3. Thodey K, Smolke CD. Cell biology. Bringing it together with RNA. Science.2011 Jul 22;333(6041):412-3. PubMed PMID: 21778388.
  4. Schaffer RJ, Friel EN, Souleyre EJ, Bolitho K, Thodey K, Ledger S, et al. A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway. Plant Physiol. 2007;144(4):1899-912. PMCID: 1949883.
  5. Janssen BJ, Thodey K, Schaffer RJ, Alba R, Balakrishnan L, Bishop R, et al. Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biol. 2008;8:16. PMCID: 2287172.
  6. Walton EF, Wu RM, Richardson AC, Davy M, Hellens RP, Thodey K, et al. A rapid transcriptional activation is induced by the dormancy-breaking chemical hydrogen cyanamide in kiwifruit (Actinidia deliciosa) buds. J Exp Bot. 2009;60(13):3835-48. PMCID: 2736901.
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