User:Kate Thodey - Revision history

Kate Thodey: /* Research interests */

Kate Thodey — Mon, 13 Aug 2012 14:32:27 GMT

Research interests

←Older revision		Revision as of 14:32, 13 August 2012
Line 13:		Line 13:

	==Research interests==		==Research interests==
-	Many drugs are still produced the traditional way; by extraction from medicinal plants. In many cases the alternative chemical syntheses of such compounds are low-yielding due to the complex nature of these biological molecules. In the Smolke laboratory we are interested in using new synthetic biology approaches to biosynthesize a class of drug molecules which are currently produced by extraction from opium poppies, namely the benzylisoquinoline alkaloids which include the powerful analgesics codeine and morphine. As a postdoc working together with members of the laboratory, my research focuses on metabolically engineering yeast to produce ~~opiates~~ and other high-value alkaloids.	+	Many drugs are still produced the traditional way; by extraction from medicinal plants. In many cases the alternative chemical syntheses of such compounds are low-yielding due to the complex nature of these biological molecules. In the Smolke laboratory we are interested in using new synthetic biology approaches to biosynthesize a class of drug molecules which are currently produced by extraction from opium poppies, namely the benzylisoquinoline alkaloids which include the powerful analgesics codeine and morphine. As a postdoc working together with members of the laboratory, my research focuses on metabolically engineering yeast to produce opioids and other high-value alkaloids.

	==Publications==		==Publications==

Kate Thodey: /* Research interests */

Kate Thodey — Mon, 13 Aug 2012 01:39:52 GMT

Research interests

←Older revision		Revision as of 01:39, 13 August 2012
Line 13:		Line 13:

	==Research interests==		==Research interests==
-	Many drugs are still produced the traditional way; by extraction from medicinal plants. In many cases the alternative chemical syntheses of such compounds are low-yielding due to the complex nature of these biological molecules. In the Smolke laboratory we are interested in using new synthetic biology approaches to biosynthesize a class of drug molecules which are currently produced by extraction from opium poppies, namely the benzylisoquinoline alkaloids which include the powerful analgesics codeine and morphine. As a ~~Postdoc~~ working together with members of the laboratory, my research focuses on metabolically engineering yeast to produce opiates and other high-value alkaloids.	+	Many drugs are still produced the traditional way; by extraction from medicinal plants. In many cases the alternative chemical syntheses of such compounds are low-yielding due to the complex nature of these biological molecules. In the Smolke laboratory we are interested in using new synthetic biology approaches to biosynthesize a class of drug molecules which are currently produced by extraction from opium poppies, namely the benzylisoquinoline alkaloids which include the powerful analgesics codeine and morphine. As a postdoc working together with members of the laboratory, my research focuses on metabolically engineering yeast to produce opiates and other high-value alkaloids.

	==Publications==		==Publications==

Kate Thodey: /* Research interests */

Kate Thodey — Mon, 13 Aug 2012 01:38:27 GMT

Research interests

←Older revision		Revision as of 01:38, 13 August 2012
Line 13:		Line 13:

	==Research interests==		==Research interests==
-	Many drugs are still produced the traditional way, by extraction from medicinal plants. In the Smolke laboratory we are ~~working~~ to ~~take~~ a drug ~~biosynthetic pathway~~ from ~~a medicinal crop – the~~ opium ~~poppy – and engineer~~ the ~~production of~~ the ~~same therapeutic molecules in an industrial microbe, yeast~~.	+	Many drugs are still produced the traditional way; by extraction from medicinal plants. In many cases the alternative chemical syntheses of such compounds are low-yielding due to the complex nature of these biological molecules. In the Smolke laboratory we are interested in using new synthetic biology approaches to biosynthesize a class of drug molecules which are currently produced by extraction from opium poppies, namely the benzylisoquinoline alkaloids which include the powerful analgesics codeine and morphine. As a Postdoc working together with members of the laboratory, my research focuses on metabolically engineering yeast to produce opiates and other high-value alkaloids.
-		+
-	As a Postdoc working together with members of the ~~Metabolic Engineering team~~, my research focuses ~~broadly~~ on metabolically-engineering yeast to produce opiate alkaloids such as morphine and codeine. In order to maximize the number and quantity of morphinan alkaloids we can produce by microbial fermentation, my approach is to focus on the spatial engineering of this pathway in the host yeast cell. Spatial engineering is particularly useful in this instance because in the natural system, the opium poppy, the biosynthesis of opiates ~~takes place across several cell types~~ and ~~subcellular structures. It could therefore be expected that efforts to engineer this pathway within single~~-celled yeast would benefit from making use of the available cellular compartments. Yeast organelles could provide microenvironments that are conducive to opiate alkaloid biosynthesis due to optimal pH or the availability of cofactors; they could compartmentalize toxic metabolites or ensure a local concentration of reactive intermediates; and they may be used to support the reconstitution of enzyme complexes which form naturally in the opium poppy. In the future, we envisage that the development of yeast synthetic organelles could support further spatial engineering efforts without taxing the functioning of native organelles.	+

	==Publications==		==Publications==

Kate Thodey: /* Publications */

Kate Thodey — Sat, 10 Mar 2012 00:01:18 GMT

Publications

←Older revision		Revision as of 00:01, 10 March 2012
Line 18:		Line 18:

	==Publications==		==Publications==
-	#Siddiqui MS, Thodey K, Trenchard I, Smolke CD. Advancing secondary metabolite	+	#Siddiqui MS, Thodey K, Trenchard I, Smolke CD. Advancing secondary metabolite biosynthesis in yeast with synthetic biology tools. FEMS Yeast Res. 2012 Mar;12(2):144-70. doi: 10.1111/j.1567-1364.2011.00774.x. Epub 2012 Jan 11. PubMed PMID: 22136110.
-	biosynthesis in yeast with synthetic biology tools. FEMS Yeast Res. 2012	+	#Michener JK, Thodey K, Liang JC, Smolke CD. Applications of genetically-encoded biosensors for the construction and control of biosynthetic pathways. Metab Eng. 2011 Sep 18. [Epub ahead of print] PubMed PMID: 21946159; PubMed Central PMCID: PMC3256257.
-	Mar;12(2):144-70. doi: 10.1111/j.1567-1364.2011.00774.x. Epub 2012 Jan 11. PubMed	+	# Thodey K, Smolke CD. Cell biology. Bringing it together with RNA. Science.2011 Jul 22;333(6041):412-3. PubMed PMID: 21778388.
-	PMID: 22136110.	+
-	#Michener JK, Thodey K, Liang JC, Smolke CD. Applications of	+
-	genetically-encoded biosensors for the construction and control of biosynthetic	+
-	pathways. Metab Eng. 2011 Sep 18. [Epub ahead of print] PubMed PMID: 21946159;	+
-	PubMed Central PMCID: PMC3256257.	+
-	#Thodey K, Smolke CD. Cell biology. Bringing it together with RNA. Science.	+
-	2011 Jul 22;333(6041):412-3. PubMed PMID: 21778388.	+
	# Schaffer RJ, Friel EN, Souleyre EJ, Bolitho K, Thodey K, Ledger S, et al. A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway. Plant Physiol. 2007;144(4):1899-912. PMCID: 1949883.		# Schaffer RJ, Friel EN, Souleyre EJ, Bolitho K, Thodey K, Ledger S, et al. A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway. Plant Physiol. 2007;144(4):1899-912. PMCID: 1949883.
	# Janssen BJ, Thodey K, Schaffer RJ, Alba R, Balakrishnan L, Bishop R, et al. Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biol. 2008;8:16. PMCID: 2287172.		# Janssen BJ, Thodey K, Schaffer RJ, Alba R, Balakrishnan L, Bishop R, et al. Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biol. 2008;8:16. PMCID: 2287172.
	# Walton EF, Wu RM, Richardson AC, Davy M, Hellens RP, Thodey K, et al. A rapid transcriptional activation is induced by the dormancy-breaking chemical hydrogen cyanamide in kiwifruit (Actinidia deliciosa) buds. J Exp Bot. 2009;60(13):3835-48. PMCID: 2736901.		# Walton EF, Wu RM, Richardson AC, Davy M, Hellens RP, Thodey K, et al. A rapid transcriptional activation is induced by the dormancy-breaking chemical hydrogen cyanamide in kiwifruit (Actinidia deliciosa) buds. J Exp Bot. 2009;60(13):3835-48. PMCID: 2736901.

Kate Thodey: /* Publications */

Kate Thodey — Fri, 09 Mar 2012 23:58:38 GMT

Publications

←Older revision		Revision as of 23:58, 9 March 2012
Line 18:		Line 18:

	==Publications==		==Publications==
		+	#Siddiqui MS, Thodey K, Trenchard I, Smolke CD. Advancing secondary metabolite
		+	biosynthesis in yeast with synthetic biology tools. FEMS Yeast Res. 2012
		+	Mar;12(2):144-70. doi: 10.1111/j.1567-1364.2011.00774.x. Epub 2012 Jan 11. PubMed
		+	PMID: 22136110.
		+	#Michener JK, Thodey K, Liang JC, Smolke CD. Applications of
		+	genetically-encoded biosensors for the construction and control of biosynthetic
		+	pathways. Metab Eng. 2011 Sep 18. [Epub ahead of print] PubMed PMID: 21946159;
		+	PubMed Central PMCID: PMC3256257.
		+	#Thodey K, Smolke CD. Cell biology. Bringing it together with RNA. Science.
		+	2011 Jul 22;333(6041):412-3. PubMed PMID: 21778388.
	# Schaffer RJ, Friel EN, Souleyre EJ, Bolitho K, Thodey K, Ledger S, et al. A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway. Plant Physiol. 2007;144(4):1899-912. PMCID: 1949883.		# Schaffer RJ, Friel EN, Souleyre EJ, Bolitho K, Thodey K, Ledger S, et al. A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway. Plant Physiol. 2007;144(4):1899-912. PMCID: 1949883.
	# Janssen BJ, Thodey K, Schaffer RJ, Alba R, Balakrishnan L, Bishop R, et al. Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biol. 2008;8:16. PMCID: 2287172.		# Janssen BJ, Thodey K, Schaffer RJ, Alba R, Balakrishnan L, Bishop R, et al. Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biol. 2008;8:16. PMCID: 2287172.
	# Walton EF, Wu RM, Richardson AC, Davy M, Hellens RP, Thodey K, et al. A rapid transcriptional activation is induced by the dormancy-breaking chemical hydrogen cyanamide in kiwifruit (Actinidia deliciosa) buds. J Exp Bot. 2009;60(13):3835-48. PMCID: 2736901.		# Walton EF, Wu RM, Richardson AC, Davy M, Hellens RP, Thodey K, et al. A rapid transcriptional activation is induced by the dormancy-breaking chemical hydrogen cyanamide in kiwifruit (Actinidia deliciosa) buds. J Exp Bot. 2009;60(13):3835-48. PMCID: 2736901.

Kate Thodey: /* Contact Info */

Kate Thodey — Fri, 09 Mar 2012 23:57:24 GMT

Contact Info

←Older revision		Revision as of 23:57, 9 March 2012
Line 1:		Line 1:
		+	{{Smolke_Top}}
	==Contact Info==		==Contact Info==
	[[Image:Kate_Thodey.jpg\|right\|thumb]]		[[Image:Kate_Thodey.jpg\|right\|thumb]]

Kate Thodey: /* Contact Info */

Kate Thodey — Tue, 24 May 2011 18:56:06 GMT

Contact Info

←Older revision		Revision as of 18:56, 24 May 2011
Line 1:		Line 1:
	==Contact Info==		==Contact Info==
-	[[Image:~~OWWEmblem~~.~~png\|thumb~~\|right\|~~Kate Thodey (an artistic interpretation)~~]]	+	[[Image:Kate_Thodey.jpg\|right\|thumb]]

	Kate Thodey<br>		Kate Thodey<br>

Josh K. Michener at 21:15, 2 March 2011

Josh K. Michener — Wed, 02 Mar 2011 21:15:27 GMT

←Older revision		Revision as of 21:15, 2 March 2011
Line 1:		Line 1:
-
-
	==Contact Info==		==Contact Info==
	[[Image:OWWEmblem.png\|thumb\|right\|Kate Thodey (an artistic interpretation)]]		[[Image:OWWEmblem.png\|thumb\|right\|Kate Thodey (an artistic interpretation)]]
Line 10:		Line 8:

	==Education==		==Education==
-	~~<!--Include info about your educational background-->~~
	* 2005-2009, PhD, John Innes Centre, Norwich, United Kingdom		* 2005-2009, PhD, John Innes Centre, Norwich, United Kingdom
	* 2000-2003, BSc(Hons), University of Auckland, Auckland, New Zealand		* 2000-2003, BSc(Hons), University of Auckland, Auckland, New Zealand

	==Research interests==		==Research interests==
-	Many drugs are still produced the traditional way; by extraction from medicinal plants. In the Smolke laboratory we are working to take a drug biosynthetic pathway from a medicinal crop – the opium poppy – and engineer the production of the same therapeutic molecules in an industrial microbe; yeast.~~<br>~~	+	Many drugs are still produced the traditional way, by extraction from medicinal plants. In the Smolke laboratory we are working to take a drug biosynthetic pathway from a medicinal crop – the opium poppy – and engineer the production of the same therapeutic molecules in an industrial microbe, yeast.
-	~~<br>~~	+
	As a Postdoc working together with members of the Metabolic Engineering team, my research focuses broadly on metabolically-engineering yeast to produce opiate alkaloids such as morphine and codeine. In order to maximize the number and quantity of morphinan alkaloids we can produce by microbial fermentation, my approach is to focus on the spatial engineering of this pathway in the host yeast cell. Spatial engineering is particularly useful in this instance because in the natural system, the opium poppy, the biosynthesis of opiates takes place across several cell types and subcellular structures. It could therefore be expected that efforts to engineer this pathway within single-celled yeast would benefit from making use of the available cellular compartments. Yeast organelles could provide microenvironments that are conducive to opiate alkaloid biosynthesis due to optimal pH or the availability of cofactors; they could compartmentalize toxic metabolites or ensure a local concentration of reactive intermediates; and they may be used to support the reconstitution of enzyme complexes which form naturally in the opium poppy. In the future, we envisage that the development of yeast synthetic organelles could support further spatial engineering efforts without taxing the functioning of native organelles.		As a Postdoc working together with members of the Metabolic Engineering team, my research focuses broadly on metabolically-engineering yeast to produce opiate alkaloids such as morphine and codeine. In order to maximize the number and quantity of morphinan alkaloids we can produce by microbial fermentation, my approach is to focus on the spatial engineering of this pathway in the host yeast cell. Spatial engineering is particularly useful in this instance because in the natural system, the opium poppy, the biosynthesis of opiates takes place across several cell types and subcellular structures. It could therefore be expected that efforts to engineer this pathway within single-celled yeast would benefit from making use of the available cellular compartments. Yeast organelles could provide microenvironments that are conducive to opiate alkaloid biosynthesis due to optimal pH or the availability of cofactors; they could compartmentalize toxic metabolites or ensure a local concentration of reactive intermediates; and they may be used to support the reconstitution of enzyme complexes which form naturally in the opium poppy. In the future, we envisage that the development of yeast synthetic organelles could support further spatial engineering efforts without taxing the functioning of native organelles.

	==Publications==		==Publications==
-	~~<!-- Replace~~ the ~~PubMed ID's~~ (~~"pmid=#~~##~~####") below with~~ the ~~PubMed ID's for your publications~~. ~~You can add or remove lines as needed~~ -->	+	# Schaffer RJ, Friel EN, Souleyre EJ, Bolitho K, Thodey K, Ledger S, et al. A genomics approach reveals that aroma production in apple is controlled by ethylene predominantly at the final step in each biosynthetic pathway. Plant Physiol. 2007;144(4):1899-912. PMCID: 1949883.
-	~~<biblio>~~	+	# Janssen BJ, Thodey K, Schaffer RJ, Alba R, Balakrishnan L, Bishop R, et al. Global gene expression analysis of apple fruit development from the floral bud to ripe fruit. BMC Plant Biol. 2008;8:16. PMCID: 2287172.
-		+	# Walton EF, Wu RM, Richardson AC, Davy M, Hellens RP, Thodey K, et al. A rapid transcriptional activation is induced by the dormancy-breaking chemical hydrogen cyanamide in kiwifruit (Actinidia deliciosa) buds. J Exp Bot. 2009;60(13):3835-48. PMCID: 2736901.
-	~~// leave a comment about a paper here~~	+
-		+
-	~~</biblio>~~	+
-		+
-	~~==Useful links==~~	+
-	*[[OpenWetWare:~~Welcome\|Introductory tutorial]]~~	+
-	*[[Help\|OpenWetWare help pages]]	+

Kate Thodey: /* Research interests */

Kate Thodey — Wed, 02 Mar 2011 21:11:05 GMT

Research interests

←Older revision		Revision as of 21:11, 2 March 2011
Line 17:		Line 17:
	Many drugs are still produced the traditional way; by extraction from medicinal plants. In the Smolke laboratory we are working to take a drug biosynthetic pathway from a medicinal crop – the opium poppy – and engineer the production of the same therapeutic molecules in an industrial microbe; yeast.<br>		Many drugs are still produced the traditional way; by extraction from medicinal plants. In the Smolke laboratory we are working to take a drug biosynthetic pathway from a medicinal crop – the opium poppy – and engineer the production of the same therapeutic molecules in an industrial microbe; yeast.<br>
	<br>		<br>
-	As a Postdoc working together with members of the Metabolic Engineering team, my research focuses broadly on metabolically-engineering yeast to produce opiate alkaloids such as morphine and codeine. In order to maximize the number and quantity of morphinan alkaloids we can produce by microbial fermentation, my approach is to focus on the spatial engineering of this pathway in the host yeast cell. Spatial engineering is particularly useful in this instance because in the natural system the biosynthesis of opiates takes place across several cell types and subcellular structures ~~in the opium poppy~~. It could therefore be expected that efforts to engineer this pathway within single-celled yeast would benefit from making use of the available cellular compartments. Yeast organelles could provide microenvironments that are conducive to opiate alkaloid biosynthesis due to optimal pH or the availability of cofactors; they could compartmentalize toxic metabolites or ensure a local concentration of reactive intermediates; and they may be used to support the reconstitution of enzyme complexes which form naturally in the opium poppy. In the future, we envisage that the development of yeast synthetic organelles could support further spatial engineering efforts without taxing the functioning of native organelles.	+	As a Postdoc working together with members of the Metabolic Engineering team, my research focuses broadly on metabolically-engineering yeast to produce opiate alkaloids such as morphine and codeine. In order to maximize the number and quantity of morphinan alkaloids we can produce by microbial fermentation, my approach is to focus on the spatial engineering of this pathway in the host yeast cell. Spatial engineering is particularly useful in this instance because in the natural system, the opium poppy, the biosynthesis of opiates takes place across several cell types and subcellular structures. It could therefore be expected that efforts to engineer this pathway within single-celled yeast would benefit from making use of the available cellular compartments. Yeast organelles could provide microenvironments that are conducive to opiate alkaloid biosynthesis due to optimal pH or the availability of cofactors; they could compartmentalize toxic metabolites or ensure a local concentration of reactive intermediates; and they may be used to support the reconstitution of enzyme complexes which form naturally in the opium poppy. In the future, we envisage that the development of yeast synthetic organelles could support further spatial engineering efforts without taxing the functioning of native organelles.

	==Publications==		==Publications==

Kate Thodey: /* Research interests */

Kate Thodey — Wed, 02 Mar 2011 21:06:44 GMT

Research interests

←Older revision		Revision as of 21:06, 2 March 2011
Line 17:		Line 17:
	Many drugs are still produced the traditional way; by extraction from medicinal plants. In the Smolke laboratory we are working to take a drug biosynthetic pathway from a medicinal crop – the opium poppy – and engineer the production of the same therapeutic molecules in an industrial microbe; yeast.<br>		Many drugs are still produced the traditional way; by extraction from medicinal plants. In the Smolke laboratory we are working to take a drug biosynthetic pathway from a medicinal crop – the opium poppy – and engineer the production of the same therapeutic molecules in an industrial microbe; yeast.<br>
	<br>		<br>
-	As a Postdoc working together with members of the Metabolic Engineering team, my research focuses broadly on metabolically-engineering yeast to produce opiate alkaloids such as morphine and codeine. ~~The approach I am taking~~ to maximize the number and quantity of morphinan alkaloids we can produce by microbial fermentation is to focus on the spatial engineering of this pathway in the host yeast cell. ~~The spatial~~ engineering ~~approach~~ is particularly useful in this instance because the biosynthesis of opiates takes place across several cell types and subcellular structures in the opium poppy. ~~To successfully~~ engineer this pathway within single-celled yeast ~~we need to make~~ use of the available cellular compartments. Yeast organelles could provide microenvironments that are conducive to opiate alkaloid biosynthesis due to optimal pH or the availability of cofactors; they could compartmentalize toxic metabolites or ensure a local concentration of reactive intermediates; and they may be used to support the reconstitution of enzyme complexes which form naturally in the opium poppy. In the future, we envisage that the development of yeast synthetic organelles could support further spatial engineering efforts without taxing the functioning of native organelles.	+	As a Postdoc working together with members of the Metabolic Engineering team, my research focuses broadly on metabolically-engineering yeast to produce opiate alkaloids such as morphine and codeine. In order to maximize the number and quantity of morphinan alkaloids we can produce by microbial fermentation, my approach is to focus on the spatial engineering of this pathway in the host yeast cell. Spatial engineering is particularly useful in this instance because in the natural system the biosynthesis of opiates takes place across several cell types and subcellular structures in the opium poppy. It could therefore be expected that efforts to engineer this pathway within single-celled yeast would benefit from making use of the available cellular compartments. Yeast organelles could provide microenvironments that are conducive to opiate alkaloid biosynthesis due to optimal pH or the availability of cofactors; they could compartmentalize toxic metabolites or ensure a local concentration of reactive intermediates; and they may be used to support the reconstitution of enzyme complexes which form naturally in the opium poppy. In the future, we envisage that the development of yeast synthetic organelles could support further spatial engineering efforts without taxing the functioning of native organelles.

	==Publications==		==Publications==