User:Kathmc: Difference between revisions

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== Bio ==
#REDIRECT [[Kathleen McGinness]]
*Postdoc in the [http://web.mit.edu/biology/sauerlab/ Sauer Lab] at [http://web.mit.edu/ MIT]
 
*Ph.D. in Chemistry, [http://www.scripps.edu/e_index.html The Scripps Research Institute], 2001 (with [http://www.scripps.edu/mb/joyce/joycelab.html Gerald Joyce])
 
*B.S. in Chemistry, [http://www.umd.edu University of Maryland College Park], 1996
 
*B.S. in Psychology, University of Maryland College Park, 1996
 
 
== Science stuff I know something about... ==
 
*''in vitro'' RNA techniques
 
*''in vitro'' selection and evolution of nucleic acids
 
*generation of nucleic acid and protein libraries
 
*nucleic acid-protein interactions
 
*fishing experiments to look for things that interact with your RNA/DNA/protein of interest
 
*PCR (even colony PCR, although it is '''not''' a separate skill)
 
*bacterial protein expression/purification
 
*''in vivo'' assays with ''E. coli'' i.e. analyzing effects on proteins/RNA
 
*molecular cloning
 
*Western/Northern/Southern blot analysis
 
*oligonucleotide synthesis
 
*RNA/DNA sequencing
 
*structural probing of RNA/DNA
 
*gels, all kinds of gels: agarose, native acrylamide,  denaturing acrylamide, SDS-acrylamide, two-dimensional
 
*enzyme kinetics
 
*radiolabeling nucleic acids and proteins
 
*tmRNA (or SsrA/10Sa RNA for you purists)
 
*translation
 
*energy-dependent proteolysis in bacteria
 
*The RNA World
 
*T7 RNA polymerase promoter preferences ''in vitro''
 
*molecular biology in a world without kits
 
 
=="Personal"==
 
*My new favorite website detailing an alternative theory to evolution ([http://www.venganza.org/ WWFSMD?]).
 
*Lab-related pet peeves (partial list...)
**beeping timers
**people from other labs that come in asking for something and don't introduce themselves first (is it so hard to say,"Hi, I'm <insert name here> from the <insert PI name here> lab"??)
 
 
[[Sauer Lab| Back to the Sauer lab]]

Latest revision as of 20:25, 19 September 2005

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