User:Kathmc

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#REDIRECT [[Kathleen McGinness]]
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==Education/Work Experience==
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*Postdoc in the [http://web.mit.edu/biology/sauerlab/ Sauer Lab] at [http://web.mit.edu/ MIT]
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*Ph.D. in Chemistry, [http://www.scripps.edu/e_index.html The Scripps Research Institute], 2001 (with [http://www.scripps.edu/mb/joyce/joycelab.html Gerald Joyce])
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*B.S. in Chemistry, [http://www.umd.edu University of Maryland College Park], 1996
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*B.S. in Psychology, University of Maryland College Park, 1996
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== Science stuff I know something about... ==
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*''in vitro'' RNA techniques
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*''in vitro'' selection and evolution of nucleic acids
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*generation of nucleic acid and protein libraries
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*nucleic acid-protein interactions
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*fishing experiments to look for things that interact with your RNA/DNA/protein of interest
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*PCR (even colony PCR, although it is '''not''' a separate skill)
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*bacterial protein expression/purification
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*''in vivo'' assays with ''E. coli'' i.e. analyzing effects on proteins/RNA
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*molecular cloning
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*Western/Northern/Southern blot analysis
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*oligonucleotide synthesis
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*RNA/DNA sequencing
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*structural probing of RNA/DNA
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*gels, all kinds of gels: agarose, native acrylamide,  denaturing acrylamide, SDS-acrylamide, two-dimensional
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*enzyme kinetics
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*radiolabeling nucleic acids and proteins
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*tmRNA (or SsrA/10Sa RNA for you purists)
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*translation
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*energy-dependent proteolysis in bacteria
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*The RNA World
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*T7 RNA polymerase promoter preferences ''in vitro''
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*molecular biology in a world without kits
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=Publications=
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'''Ribosomal protein S1 binds mRNA and tmRNA similarly but plays distinct roles in translation of these molecules.'''
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McGinness, K.E. & Sauer, R.T. (2004) Proc. Natl. Acad. Sci. USA 101, 13454–13459.
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'''A Glimpse into tmRNA-mediated Ribosome Rescue.'''
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Moore, S.D., McGinness, K.E. & Sauer, R.T. (2003)  Science 300, 127–130. (comment)
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'''In Search of an RNA Replicase Ribozyme.'''
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McGinness, K.E. & Joyce, G.F. (2003)  Chem. Biol. 10, 5–14. (review)
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'''Continuous In Vitro Evolution of a Ribozyme that Catalyzes Three Successive Nucleotidyl Addition Reactions.'''
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McGinness, K.E., Wright, M.C. & Joyce, G.F. (2002)  Chem. Biol. 9, 585–596.
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'''RNA-Catalyzed RNA Ligation on an External RNA Template.'''
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McGinness, K.E. & Joyce, G.F. (2002)  Chem. Biol. 9, 297–307.
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'''Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element.'''
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McGinness, K.E. & Joyce, G.F. (2002)  J. Biol. Chem. 277, 2987–2991.
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=="Personal"==
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===Bio===
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I'm a fourth (at least!) generation Marylander who grew up in the happy Washington, D.C. suburb of Derwood. I began my college career majoring in Biology at [http://www.psu.edu/ Penn State], but after realizing that the bustling metropolis of State College was not for me, I transferred to the University of Maryland. When I started at Maryland, I realized that although I support the need for animal research, I don't have the stomach for it. So, I decided to change my major to Chemistry, and threw in a major in Psychology for good measure. While at Maryland, I had the opportunity to work in the Laboratory of Neurochemistry and the Laboratory of General and Comparative Biochemistry at [http://www.nimh.nih.gov/ NIMH] (yes, where the rats are from).  I also got my first introduction into the RNA world by working in [http://biophysics.jhu.edu/faculty-pages/woodson.html Sarah Woodson's lab] while she was at Maryland. After four years (yes, that makes it a 5 year plan for college!), I packed up my worldly possessions and headed to sunny So. Cal. to do my graduate work at Scripps with Jerry Joyce, solidifying my love of the RNA world. Much like [[user:skosuri|Sr]]i, I fell in love with CA. So, after completing my Ph.D. in chemistry (biochemistry, really), I returned to the east coast to work at MIT. In Bob Sauer's lab I have expanded my science into the protein world, but I'm still an RNA girl at heart. When I get the time, I'll put up some of my current research here.
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===Random Stuff===
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*My new favorite website detailing an alternative theory to evolution ([http://www.venganza.org/ WWFSMD?]).
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*Lab-related pet peeves (partial list...)
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**beeping timers
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**people from other labs that come in asking for something and don't introduce themselves first (is it so hard to say,"Hi, I'm <insert name here> from the <insert PI name here> lab"??)
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*I have the messiest bench (and desk) in the lab. Paradoxically, I am a tyrant about cleanliness in common lab areas.
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*[[KM's soapbox]]
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[[Sauer Lab| Back to the Sauer lab]]
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Current revision

  1. REDIRECT Kathleen McGinness
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