User:Kathmc

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Bio)
Line 1: Line 1:
== Bio ==
== Bio ==
-
Postdoc in the [http://web.mit.edu/biology/sauerlab/ Sauer Lab] at [http://web.mit.edu/ MIT]
+
*Postdoc in the [http://web.mit.edu/biology/sauerlab/ Sauer Lab] at [http://web.mit.edu/ MIT]
-
B.S. in Chemistry, [http://www.umd.edu University of Maryland College Park], 1996
+
*Ph.D. in Chemistry, [http://www.scripps.edu/e_index.html The Scripps Research Institute], 2001 (with [http://www.scripps.edu/mb/joyce/joycelab.html Gerald Joyce])
-
B.S. in Psychology, University of Maryland College Park, 1996
+
*B.S. in Chemistry, [http://www.umd.edu University of Maryland College Park], 1996
-
 
+
-
Ph.D. in Chemistry, [http://www.scripps.edu/e_index.html The Scripps Research Institute], 2001 (with [http://www.scripps.edu/mb/joyce/joycelab.html Gerald Joyce])
+
 +
*B.S. in Psychology, University of Maryland College Park, 1996
== Science stuff I know something about... ==
== Science stuff I know something about... ==

Revision as of 22:03, 15 June 2005

Bio

  • B.S. in Psychology, University of Maryland College Park, 1996

Science stuff I know something about...

  • in vitro RNA techniques
  • in vitro selection and evolution of nucleic acids
  • generation of nucleic acid and protein libraries
  • nucleic acid-protein interactions
  • fishing experiments to look for things that interact with your RNA/DNA/protein of interest
  • PCR (even colony PCR, although it is not a separate skill)
  • bacterial protein expression/purification
  • in vivo assays with E. coli i.e. analyzing effects on proteins/RNA
  • molecular cloning
  • Western/Northern/Southern blot analysis
  • oligonucleotide synthesis
  • RNA/DNA sequencing
  • structural probing of RNA/DNA
  • gels, all kinds of gels: agarose, native acrylamide, denaturing acrylamide, SDS-acrylamide, two-dimensional
  • enzyme kinetics
  • tmRNA (or SsrA/10Sa RNA for you purists)
  • translation
  • energy-dependent proteolysis in bacteria
  • The RNA World
  • T7 RNA polymerase promoter preferences in vitro
  • molecular biology in a world without kits
  • radiolabeling nucleic acids and proteins
Personal tools