User:Kathmc: Difference between revisions
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== Bio == | == Bio == | ||
Postdoc in the [http://web.mit.edu/biology/sauerlab/ Sauer Lab] at [http://web.mit.edu/ MIT] | *Postdoc in the [http://web.mit.edu/biology/sauerlab/ Sauer Lab] at [http://web.mit.edu/ MIT] | ||
*Ph.D. in Chemistry, [http://www.scripps.edu/e_index.html The Scripps Research Institute], 2001 (with [http://www.scripps.edu/mb/joyce/joycelab.html Gerald Joyce]) | |||
B.S | *B.S. in Chemistry, [http://www.umd.edu University of Maryland College Park], 1996 | ||
*B.S. in Psychology, University of Maryland College Park, 1996 | |||
== Science stuff I know something about... == | == Science stuff I know something about... == | ||
Revision as of 19:03, 15 June 2005
Bio
- Ph.D. in Chemistry, The Scripps Research Institute, 2001 (with Gerald Joyce)
- B.S. in Chemistry, University of Maryland College Park, 1996
- B.S. in Psychology, University of Maryland College Park, 1996
Science stuff I know something about...
- in vitro RNA techniques
- in vitro selection and evolution of nucleic acids
- generation of nucleic acid and protein libraries
- nucleic acid-protein interactions
- fishing experiments to look for things that interact with your RNA/DNA/protein of interest
- PCR (even colony PCR, although it is not a separate skill)
- bacterial protein expression/purification
- in vivo assays with E. coli i.e. analyzing effects on proteins/RNA
- molecular cloning
- Western/Northern/Southern blot analysis
- oligonucleotide synthesis
- RNA/DNA sequencing
- structural probing of RNA/DNA
- gels, all kinds of gels: agarose, native acrylamide, denaturing acrylamide, SDS-acrylamide, two-dimensional
- enzyme kinetics
- tmRNA (or SsrA/10Sa RNA for you purists)
- translation
- energy-dependent proteolysis in bacteria
- The RNA World
- T7 RNA polymerase promoter preferences in vitro
- molecular biology in a world without kits
- radiolabeling nucleic acids and proteins
