User:Kathmc

Bio

Postdoc in the Sauer Lab at MIT

B.S. in Chemistry, University of Maryland College Park, 1996

B.S. in Psychology, University of Maryland College Park, 1996

Ph.D. in Chemistry, The Scripps Research Institute, 2001 (with Gerald Joyce)

Science stuff I know something about...

• in vitro RNA techniques

• in vitro selection and evolution of nucleic acids

• generation of nucleic acid and protein libraries

• nucleic acid-protein interactions

• fishing experiments to look for things that interact with your RNA/DNA/protein of interest

• PCR (even colony PCR, although it is not a separate skill)

• bacterial protein expression/purification

• in vivo assays with E. coli i.e. analyzing effects on proteins/RNA

• molecular cloning

• Western/Northern/Southern blot analysis

• oligonucleotide synthesis

• RNA/DNA sequencing

• structural probing of RNA/DNA

• gels, all kinds of gels: agarose, native acrylamide, denaturing acrylamide, SDS-acrylamide, two-dimensional

• enzyme kinetics

• tmRNA (or SsrA/10Sa RNA for you purists)

• translation

• energy-dependent proteolysis in bacteria

• The RNA World

• T7 RNA polymerase promoter preferences in vitro

• molecular biology in a world without kits