User:Kathmc

Bio

• Postdoc in the Sauer Lab at MIT

• Ph.D. in Chemistry, The Scripps Research Institute, 2001 (with Gerald Joyce)

• B.S. in Chemistry, University of Maryland College Park, 1996

• B.S. in Psychology, University of Maryland College Park, 1996

Science stuff I know something about...

• in vitro RNA techniques

• in vitro selection and evolution of nucleic acids

• generation of nucleic acid and protein libraries

• nucleic acid-protein interactions

• fishing experiments to look for things that interact with your RNA/DNA/protein of interest

• PCR (even colony PCR, although it is not a separate skill)

• bacterial protein expression/purification

• in vivo assays with E. coli i.e. analyzing effects on proteins/RNA

• molecular cloning

• Western/Northern/Southern blot analysis

• oligonucleotide synthesis

• RNA/DNA sequencing

• structural probing of RNA/DNA

• gels, all kinds of gels: agarose, native acrylamide, denaturing acrylamide, SDS-acrylamide, two-dimensional

• enzyme kinetics

• radiolabeling nucleic acids and proteins

• tmRNA (or SsrA/10Sa RNA for you purists)

• translation

• energy-dependent proteolysis in bacteria

• The RNA World

• T7 RNA polymerase promoter preferences in vitro

• molecular biology in a world without kits

"Personal"

• My new favorite website detailing an alternative theory to evolution (WWFSMD?).

• Lab-related pet peeves (partial list...)
  • beeping timers
  • people from other labs that come in asking for something and don't introduce themselves first (is it so hard to say,"Hi, I'm <insert name here> from the <insert PI name here> lab"??)
• I have the messiest bench (and desk) in the lab. Paradoxically, I am a tyrant about cleanliness in common lab areas.

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