User:Kathryn A. Sloane/Notebook/Biology 210 at AU

Feb 3 2016 Bio Lab 210 Section 4 Transect 1 Group 1

Goal of the week To be able to determine different types of eukaryotic unicellular organisms (protists) using a dichotomous key and to perform serial dilutions to set up the growth of bacteria colonies.

Procedure Set up of the hay infusion-my partners and I added 10 grams of substance from our transects, this including leaves, twigs, branches, soil, acorns, and other plant life. We then added 500 ml of water and then .1 gram of dried milk powder. After that we put on the lid and shook the jar for around 60 seconds, allowing the mixture to mix together. We then let it sit for a week (with the lid off, exposed to the air) and the next class we took samples of water from near the top, middle, and bottom and put them under the microscope in order to study the different life forms existing there and determining what they were by using a dichotomous key. After that we preformed a serial dilution and set up different agar plates (based on concentration of hay infusion and type of agar plates, tet + and normal nutrient based plates).

Description of Hay Infusion Set up included adding about 10 grams of soil and other things (such as leaves and branches) from transect, adding 500ml of water, and then adding about .1 gram of dried milk powder. Then we put the lid on the jar and shook it, and then let it sit for a week without the lid on. A week later we observed the hay infusion cultures, observations: no observable organisms, gross and dingy looking, smells bad, sediment that is left has sunk to the bottom to form a sort of muck, murky, some leaves still green (though most are brown and breaking apart), a layer of film is coating the top of the hay infusion.

Protist Sample Location The protist samples came from each section of the hay infusion, one near the surface, one from the middle, one very close to the layer of "muck" at the bottom.

Top: (Oedogonium) File:Oedogonium.jpg

Middle: (Colpidium)

Bottom: (Peranma)

KS

Jan 25 2016 Bio Lab 210 Section Four Transect 1 Group 1

Abiotic Factors -Water -Wind -Soil -Rocks -Temperature

Biotic Factors -Leaves -Oak Trees -Acorns -Flowers -tree branches (sticks)

Description of Transect: Plot of land the soil is hard/compact because of the temperature (28 degrees) the soil underneath the frozen top layer is loose and cold. 11 mph winds occurring (at the time we were there 5:30-6:00pm). Three larger oak trees and several smaller trees. There were many twigs, sticks, and tree branches littering the ground. There were also clumps of decomposing leaves and fallen acorns scattered across the ground. There were some remnants of plant life that had been growing there, flowers, small bushes and patches of clover. The air was cold and the sky was dark given the time frame.

Aerial View of Transect:

KS