User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/05/24: Difference between revisions
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==Experimental Plan== | ==Experimental Plan== | ||
# [[Muratore: | # [[Muratore:Protocols/DNA purification/Miniprep| Miniprep]] BSA clone | ||
## streak clone on LB+Amp<sup>100</sup> plates - today | ## streak clone on LB+Amp<sup>100</sup> plates - today | ||
### O/N @ 37°C | ### O/N @ 37°C | ||
Line 71: | Line 71: | ||
#sequence | #sequence | ||
#Sequential double-digest | #Sequential double-digest | ||
##[[Muratore: | ##[[Muratore:Protocols/Digest/NheI| NheI digest]] | ||
##[[Muratore: | ##[[Muratore:Protocols/Digest/SapI| SapI digest]] | ||
##Phosphatase vector | ##Phosphatase vector | ||
##[[Muratore: | ##[[Muratore:Protocols/DNA purification/Gel purification| gel purify]] | ||
#[[Muratore: | #[[Muratore:Protocols/Ligation| ligate]] | ||
#[[Muratore: | #[[Muratore:Protocols/Transformation| transform]] DH10B | ||
#[[Muratore: | #[[Muratore:Protocols/DNA purification/Miniprep| miniprep]] | ||
#sequence | #sequence | ||
#express | #express | ||
==Today's benchwork== | ==Today's benchwork== | ||
# Streak out [[#BSA clone|BSA clone]] on [[Muratore: | # Streak out [[#BSA clone|BSA clone]] on [[Muratore:Materials/Media/LB| LB]]Amp<sup>100</sup> | ||
#* Plates made by TF and KW yesterday | #* Plates made by TF and KW yesterday | ||
::→ 37C O/N | ::→ 37C O/N |
Revision as of 15:07, 26 May 2011
AU CHEM-570 Lab Prep | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveSubclone BSA into pTXB1
Special MaterialsBSA clone
PCR primers
pTXB1
Experimental Plan
Today's benchwork
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