User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/05/24: Difference between revisions
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::: MW = 8946.8 g/mol | ::: MW = 8946.8 g/mol | ||
::: 16.9 nmol, 0.15 mg | ::: 16.9 nmol, 0.15 mg | ||
:*'''[[User:Kathryn Muratore|Kathryn Muratore]] 14:09, 11 August 2011 (EDT)''':This is the wrong sequence. It leaves an overhang of GCA at the 3' end, but the overhang for pTXB1 is TGC. I inverted these three bases when I designed the primer, and this explains why the ligation did not work. | |||
* His-tag cloning | * His-tag cloning | ||
** ins_His_after_CBD | ** ins_His_after_CBD |
Revision as of 11:09, 11 August 2011
AU CHEM-570 Lab Prep | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveSubclone BSA into pTXB1
Special MaterialsBSA clone
PCR primers
pTXB1
Experimental Plan
Today's benchwork
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