User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/05/25: Difference between revisions

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##10 mL [[Muratore:Material/Media/LB| LB]] + 10 μL 100 mg/mL [[Muratore:Material/Antibiotics/Amp| Amp]]
##10 mL [[Muratore:Material/Media/LB| LB]] + 10 μL 100 mg/mL [[Muratore:Material/Antibiotics/Amp| Amp]]
##*inoculate with single colony from plate in step 1
##*inoculate with single colony from plate in step 1
:&rarr 37°C O/N
:→ 37°C O/N




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Revision as of 11:29, 26 May 2011

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Objective

Isolate BSA clone DNA

Bench work

  1. (cont.) Colonies grew on plate.
→ 4°C
  1. Miniprep
    1. 10 mL LB + 10 μL 100 mg/mL Amp
      • inoculate with single colony from plate in step 1
→ 37°C O/N