User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/05/25: Difference between revisions
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==Bench work== | ==Bench work== | ||
# ([[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/05/24#Today's benchwork|cont.]]) Colonies grew on plate. | # ([[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/05/24#Today's benchwork|cont.]]) Colonies grew on plate. | ||
#*→ 4°C | |||
# Miniprep | # Miniprep | ||
##10 mL [[Muratore:Material/Media/LB| LB]] + 10 μL 100 mg/mL [[Muratore:Material/Antibiotics/Amp| Amp]] | ##10 mL [[Muratore:Material/Media/LB| LB]] + 10 μL 100 mg/mL [[Muratore:Material/Antibiotics/Amp| Amp]] | ||
##*inoculate with single colony from plate in step 1 | ##*inoculate with single colony from plate in step 1 | ||
##*→ 37°C O/N | |||
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Revision as of 11:31, 26 May 2011
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ObjectiveIsolate BSA clone DNA Bench work
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