User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/05/26: Difference between revisions
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#**5 μL plasmid + 74 μL sterile H<sub>2</sub>O | #**5 μL plasmid + 74 μL sterile H<sub>2</sub>O | ||
#*followed [[Muratore:Protocols/PCR/Basic| Basic PCR protocol]] | #*followed [[Muratore:Protocols/PCR/Basic| Basic PCR protocol]] | ||
#**template = 10 μg/mL [[Muratore:Materials/ | #**template = 10 μg/mL [[Muratore:Materials/Plasmids/pCMV-SPORT6+BSA| pCMV-SPORT6+BSA]] | ||
#**5' primer = 4 μM [[Muratore:Materials/Primers/BSA-NheI-5'| BSA-NheI-5']] | #**5' primer = 4 μM [[Muratore:Materials/Primers/BSA-NheI-5'| BSA-NheI-5']] | ||
#**3' primer = 4 μM [[Muratore:Materials/Primers/BSA-SapI-3'| BSA-SapI-3']] | #**3' primer = 4 μM [[Muratore:Materials/Primers/BSA-SapI-3'| BSA-SapI-3']] | ||
Revision as of 08:01, 2 June 2011
 AU CHEM-570 Lab Prep
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Today's Benchwork
Results[math]\displaystyle{ [CMV-SPORT6+BSA]=(0.130AU+0.028AU)\times50\frac{\frac{\mu g}{mL}}{AU}\times\frac{100\mu L}{5\mu L}=158\frac{\mu g}{mL} }[/math] [math]\displaystyle{ \frac{A_{260}}{A_{280}}=\frac{0.130AU+0.028AU}{0.055AU+0.029AU}=1.88 }[/math]
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