User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/07: Difference between revisions
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== | ==Objective== | ||
* | * Create a new empty vector that has an intein, chitin binding domain, and his-tag. | ||
==Bench work== | |||
# [[User:Kathryn Muratore/Protocols/QuikChange| QuikChange]] | |||
#* template = 10 μg/mL [[User:Kathryn Muratore/Materials/pTXB1| pTXB1]] | |||
#* forward primer = 12.5 ng/μL [[User:Kathryn Muratore/Materials/ins-His-after-CBD| ins-His-after-CBD]] | |||
#* reverse primer = 12.5 ng/μL [[User:Kathryn Muratore/Materials/Rins-His-after-CBD| Rins-His-after-CBD]] | |||
#* anneal step = 7 min | |||
#* 18 melt/anneal/extend cycles | |||
# analytical minigel | |||
# DpnI digestion | |||
#* add 1 μL DpnI to experimental and control tubes from step 1 | |||
#*→ 1h @ 37°C | |||
#*→ store at 4°C | |||
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Revision as of 19:30, 7 June 2011
 AU CHEM-570 Lab Prep
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Objective
Bench work
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