User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/07: Difference between revisions
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==Bench work== | ==Bench work== | ||
# [[ | # [[Muratore/Protocols/QuikChange| QuikChange]] | ||
#* template = 10 μg/mL [[ | #* template = 10 μg/mL [[pTXB1]] | ||
#* forward primer = 12.5 ng/μL [[ | #* forward primer = 12.5 ng/μL [[Muratore/Materials/Primers/Ins-His-after-CBD| ins-His-after-CBD]] | ||
#* reverse primer = 12.5 ng/μL [[ | #* reverse primer = 12.5 ng/μL [[Muratore/Materials/Rins-His-after-CBD| Rins-His-after-CBD]] | ||
#* anneal step = 7 min | #* anneal step = 7 min | ||
#* 18 melt/anneal/extend cycles | #* 18 melt/anneal/extend cycles | ||
# analytical minigel | # analytical minigel | ||
# DpnI digestion | # [[DpnI]] digestion | ||
#* add 1 μL DpnI to experimental and control tubes from step 1 | #* add 1 μL [[DpnI]] to experimental and control tubes from step 1 | ||
#*→ 1h @ 37°C | #*→ 1h @ 37°C | ||
#*→ store at 4°C | #*→ store at 4°C | ||
Revision as of 18:14, 8 June 2011
 AU CHEM-570 Lab Prep
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Objective
Bench work
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