User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/13: Difference between revisions
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#*→ 1h @ 37°C | #*→ 1h @ 37°C | ||
#*→ store @ -20°C | #*→ store @ -20°C | ||
# [[Transformation| Transform]] [[DH10B]] | |||
## 5μL DpnI-digested QuikChange experimental reaction + 200μL [[Muratore:Materials/Strains/DH10B#Competent cells| competent DH10B]] | |||
## 5μL DpnI-digested QuikChange control reaction + 200μL [[Muratore:Materials/Strains/DH10B#Competent cells| competent DH10B]] | |||
#* follow [[Muratore:Protocols/Transformation| standard transformation protocol]] | |||
#* plate 200μL | |||
#*→ 37°C O/N | |||
==Results== | ==Results== |
Revision as of 11:11, 14 June 2011
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Bench work
Results
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