User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/15: Difference between revisions
From OpenWetWare
Line 15: | Line 15: | ||
===Experiments=== | ===Experiments=== | ||
# Glycerol stock | # Glycerol stock | ||
#* 750μL pTXB1His/DH10B QC colony #1 culture from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/14| yesterday]] + 250 μL 60% glycerol (sterile) | #* 750μL pTXB1His/DH10B QC colony #1 culture from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/14| yesterday]] + 250 μL 60% glycerol (sterile) → [[Muratore:Materials/glycerol stocks#79| tube #79]] | ||
#*→ -80°C | #*→ -80°C | ||
# [[Muratore:Protocols/DNA purification/Miniprep| Miniprep]] of pTXB1His | # [[Muratore:Protocols/DNA purification/Miniprep| Miniprep]] of pTXB1His |
Revision as of 08:43, 7 July 2011
AU CHEM-570 Lab Prep | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |
BenchworkPlan
Experiments
ResultsTransformations
Miniprep[math]\displaystyle{ [pTXB1His\#1]=(0.114AU+0.012AU)\times50\frac{\frac{\mu g}{mL}}{AU}\times\frac{100\mu L}{5\mu L}=102\frac{\mu g}{mL} }[/math] [math]\displaystyle{ \frac{A_{260}}{A_{280}}=\frac{0.114AU+0.012AU}{0.067AU+0.011AU}=1.82 }[/math] Sequencing
{{#widget:Google Document |
id=19hEjXbw_pwVjY5vwu-hQGfqpLTuwWQemRzEsCvJw640 |