User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/15: Difference between revisions

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(Autocreate 2011/06/15 Entry for User:Kathryn_Muratore/Notebook/AU_CHEM-570_lab_prep)
 
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==Content==
==Benchwork==
* Add content here...
===Plan===
* Miniprep pTXB1His and send for sequencing
* Start more cultures of new colonies for more minipreps
* Don't forget to miniprep pTXB1 (unmodified) as well
* Streak expression strain (ER2566) on LB so competent cells and a glycerol stock can be made
 
===Experiments===
# Glycerol stock
#* 750μL pTXB1His/DH10B QuikChange colony #1 culture from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/14| yesterday]] + 250 μL 60% glycerol (sterile)
#*&rarr; -80°C
# [[Muratore:Protocols/DNA purification/Miniprep| Miniprep]] of pTXB1His
 
==Results==
* Four plates yesterday:
** 800μL experimental transformation re-plated from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/13| 2 days ago]]: 1 colony
** 800μL control transformation re-plated from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/13| 2 days ago]]: 0
** 200μL experimental transformation from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/14| yesterday]]: 1 colony
** 200μL control transformation from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/14| yesterday]]: 0


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Revision as of 08:41, 15 June 2011

AU CHEM-570 Lab Prep <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Benchwork

Plan

  • Miniprep pTXB1His and send for sequencing
  • Start more cultures of new colonies for more minipreps
  • Don't forget to miniprep pTXB1 (unmodified) as well
  • Streak expression strain (ER2566) on LB so competent cells and a glycerol stock can be made

Experiments

  1. Glycerol stock
    • 750μL pTXB1His/DH10B QuikChange colony #1 culture from yesterday + 250 μL 60% glycerol (sterile)
    • → -80°C
  2. Miniprep of pTXB1His

Results

  • Four plates yesterday:
    • 800μL experimental transformation re-plated from 2 days ago: 1 colony
    • 800μL control transformation re-plated from 2 days ago: 0
    • 200μL experimental transformation from yesterday: 1 colony
    • 200μL control transformation from yesterday: 0
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