User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/15: Difference between revisions

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==Results==
==Results==
* Four plates yesterday:
* Four plates yesterday:
** 200μL experimental transformation from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/14| yesterday]]: 1 colony
***→ pTXB1His QC colony #2
** 200μL control transformation from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/14| yesterday]]: 0
** 800μL experimental transformation re-plated from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/13| 2 days ago]]: 1 colony
** 800μL experimental transformation re-plated from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/13| 2 days ago]]: 1 colony
***→ pTXB1His QC colony #3
** 800μL control transformation re-plated from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/13| 2 days ago]]: 0
** 800μL control transformation re-plated from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/13| 2 days ago]]: 0
** 200μL experimental transformation from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/14| yesterday]]: 1 colony
** 200μL control transformation from [[User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/14| yesterday]]: 0


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Revision as of 14:43, 15 June 2011

AU CHEM-570 Lab Prep <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Benchwork

Plan

  • Miniprep pTXB1His and send for sequencing
  • Start more cultures of new colonies for more minipreps
  • Don't forget to miniprep pTXB1 (unmodified) as well
  • Streak expression strain (ER2566) on LB so competent cells and a glycerol stock can be made

Experiments

  1. Glycerol stock
    • 750μL pTXB1His/DH10B QuikChange colony #1 culture from yesterday + 250 μL 60% glycerol (sterile)
    • → -80°C
  2. Miniprep of pTXB1His

Results

  • Four plates yesterday:
    • 200μL experimental transformation from yesterday: 1 colony
      • → pTXB1His QC colony #2
    • 200μL control transformation from yesterday: 0
    • 800μL experimental transformation re-plated from 2 days ago: 1 colony
      • → pTXB1His QC colony #3
    • 800μL control transformation re-plated from 2 days ago: 0
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