User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/15: Difference between revisions
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## + 5μL miniprepped DNA from step 2 | ## + 5μL miniprepped DNA from step 2 | ||
#* → measure absorbance at 260 nm and 280 nm | #* → measure absorbance at 260 nm and 280 nm | ||
# DNA sequencing | |||
#* send pTXB1His #1 for sequencing with [[M13-Rev]] and [[M13-For]] primers | |||
#* Also sent Kay Wang's MDHI12VR81M plasmid for sequencing with [[Muratore:Materials/Primers/PKK223-3-For| pKK223-3-For]] and [[pBAD-Rev]] primers | |||
#* Sequencing is done by [http://biolchem.bs.jhmi.edu/tssf/pages/page.aspx?PageName=DNAseq| Johns Hopkins Synthesis and Sequencing Facility] | |||
# Miniprep cultures of pTXB1His and pTXB1 in DH10B | # Miniprep cultures of pTXB1His and pTXB1 in DH10B | ||
## 10mL LB + 10μL 100 mg/mL Amp + pTXB1His/DH10B QC colony #2 (see [[#Results| Results below]]) | ## 10mL LB + 10μL 100 mg/mL Amp + pTXB1His/DH10B QC colony #2 (see [[#Results| Results below]]) |
Revision as of 07:05, 20 June 2011
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BenchworkPlan
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Results
[math]\displaystyle{ [pTXB1His\#1]=(0.114AU+0.012AU)\times50\frac{\frac{\mu g}{mL}}{AU}\times\frac{100\mu L}{5\mu L}=102\frac{\mu g}{mL} }[/math] [math]\displaystyle{ \frac{A_{260}}{A_{280}}=\frac{0.114AU+0.012AU}{0.067AU+0.011AU}=1.82 }[/math] |