User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/15

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Benchwork

Plan

  • Miniprep pTXB1His and send for sequencing
  • Start more cultures of new colonies for more minipreps
  • Don't forget to miniprep pTXB1 (unmodified) as well
  • Streak expression strain (ER2566) on LB so competent cells and a glycerol stock can be made

Experiments

  1. Glycerol stock
    • 750μL pTXB1His/DH10B QuikChange colony #1 culture from yesterday + 250 μL 60% glycerol (sterile)
    • → -80°C
  2. Miniprep of pTXB1His

Results

  • Four plates yesterday:
    • 200μL experimental transformation from yesterday: 1 colony
      • → pTXB1His QC colony #2
    • 200μL control transformation from yesterday: 0
    • 800μL experimental transformation re-plated from 2 days ago: 1 colony
      • → pTXB1His QC colony #3
    • 800μL control transformation re-plated from 2 days ago: 0
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