User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/21: Difference between revisions
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;* '''[[User:Kathryn Muratore|Kathryn Muratore]] 10:22, 28 June 2011 (EDT)''': Actually, although the calculations were off by 8-fold in terms of mass, the final [DNA] was 3 higher than the upper-bounds of the recommended amount in terms of mass (1-10μg/mL), but at the low end of the recommended amount in terms of moles (0.1-1μM). Fortuitously, I think this was the right amount of DNA to use in this ligation. | ;* '''[[User:Kathryn Muratore|Kathryn Muratore]] 10:22, 28 June 2011 (EDT)''': Actually, although the calculations were off by 8-fold in terms of mass, the final [DNA] was 3 higher than the upper-bounds of the recommended amount in terms of mass (1-10μg/mL), but at the low end of the recommended amount in terms of moles (0.1-1μM). Fortuitously, I think this was the right amount of DNA to use in this ligation. | ||
;*'''[[User:Kathryn Muratore|Kathryn Muratore]] 10:08, 28 July 2011 (EDT)''': I did not subtract the background previously. This is now corrected. | |||
===First gel purification=== | ===First gel purification=== |
Revision as of 07:08, 28 July 2011
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Bench work
ResultsAnalytical minigel
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First gel purificationSecond gel purification
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