User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/23: Difference between revisions

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===Analytical gel===
===Analytical gel===
[[Image:DNA_gel_110623_annotated.jpg]]
[[Image:DNA_gel_110623_annotated.jpg]]
* Not surprising that nothing is visible in 6th lane: The double-digested pTXB1 mostly floated away.
* Not surprising that nothing is visible:  
* However, the ligation reaction lane has about 7ug total [DNA], which I would expect to be visible.
** The double-digested pTXB1 mostly floated away.
** The ligation reaction lane has about 150ng total [DNA] (the comparable bands in the ladder have ~300ng). This might be visible, but, if the estimations of [DNA] are off, then it might not be visible.


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Revision as of 10:57, 24 June 2011

AU CHEM-570 Lab Prep <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

Add today's objective(s) here...

Bench work

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Results

Plates

Analytical gel

  • Not surprising that nothing is visible:
    • The double-digested pTXB1 mostly floated away.
    • The ligation reaction lane has about 150ng total [DNA] (the comparable bands in the ladder have ~300ng). This might be visible, but, if the estimations of [DNA] are off, then it might not be visible.
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