User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/29: Difference between revisions
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===Transformation=== | ===Transformation=== | ||
[[../28| yesterday's]] plates both had of colonies. After some poking around, we realized that the Ampicillin stocks made on 6/20 are only 10 mg/mL not 100 mg/mL. The plates poured yesterday were the first batch using the new Amp and are thus LBAmp<sup>10</sup> not LBAmp<sup>100</sup>. I will be re-plating another 100μL on new LBAmp<sup>100</sup> plates today. | [[../28| yesterday's]] plates both had of colonies. After some poking around, we realized that the Ampicillin stocks made on 6/20 are only 10 mg/mL not 100 mg/mL. The plates poured yesterday were the first batch using the new Amp and are thus LBAmp<sup>10</sup> not LBAmp<sup>100</sup>. I will be re-plating another 100μL on new LBAmp<sup>100</sup> plates today. | ||
===Gel purification=== | |||
[[Image:DNA gel 110629 annotated.jpg]] | |||
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* Looks good. Equal volumes of insert and vector will be fine. [DNA] calculations are based on 8.5μL each. | |||
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Revision as of 07:35, 30 June 2011
AU CHEM-570 Lab Prep | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |
ObjectiveTry to get the ligation and or transformation to work for the BSA-intein constructs. Bench work
ResultsTransformationyesterday's plates both had of colonies. After some poking around, we realized that the Ampicillin stocks made on 6/20 are only 10 mg/mL not 100 mg/mL. The plates poured yesterday were the first batch using the new Amp and are thus LBAmp10 not LBAmp100. I will be re-plating another 100μL on new LBAmp100 plates today. Gel purification |
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