User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/29: Difference between revisions
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## 50μL NheI/SapI/Phosphatased pTXB1 + 10μL loading buffer | ## 50μL NheI/SapI/Phosphatased pTXB1 + 10μL loading buffer | ||
##* 1.2834g - 1.0295g = 253.9mg agarose → 260μL Membrane Binding Solution | ##* 1.2834g - 1.0295g = 253.9mg agarose → 260μL Membrane Binding Solution | ||
# | #* I incubated the agarose on the column for 1 minute this time (as stated in instructions) | ||
# | #* I centrifuged the washed columns "without the lid" using the microfuge and manually holding the "on" button (this is not very safe, but not dangerous if you are careful and others are not in the area.) | ||
#*→ store @ -10°C | #*→ store @ -10°C | ||
# Ligation | # Ligation |
Revision as of 08:26, 1 July 2011
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ObjectiveTry to get the ligation and or transformation to work for the BSA-intein constructs. Bench work
ResultsTransformationyesterday's plates both had of colonies. After some poking around, we realized that the Ampicillin stocks made on 6/20 are only 10 mg/mL not 100 mg/mL. The plates poured yesterday were the first batch using the new Amp and are thus LBAmp10 not LBAmp100. I will be re-plating another 100μL on new LBAmp100 plates today. Gel purification |
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