User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/29: Difference between revisions
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#** Use PCR machine as cold block | #** Use PCR machine as cold block | ||
# Re-plate transformation | # Re-plate transformation | ||
#* 100μL of [[../28| yesterday's]] transformations on 2 | #* 100μL of [[../28| yesterday's]] transformations on 2 separate LBAmp<sup>100</sup> plates (one for each ligation) | ||
#*→ 37°C O/N | #*→ 37°C O/N | ||
Revision as of 08:27, 1 July 2011
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ObjectiveTry to get the ligation and or transformation to work for the BSA-intein constructs. Bench work
ResultsTransformationyesterday's plates both had of colonies. After some poking around, we realized that the Ampicillin stocks made on 6/20 are only 10 mg/mL not 100 mg/mL. The plates poured yesterday were the first batch using the new Amp and are thus LBAmp10 not LBAmp100. I will be re-plating another 100μL on new LBAmp100 plates today. Gel purification |
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