User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/06/29: Difference between revisions
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[[Image:DNA gel 110629 annotated.jpg]] | [[Image:DNA gel 110629 annotated.jpg]] | ||
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* Looks good. Equal volumes of insert and vector will be fine. [DNA] calculations are based on 8.5μL each. | * Looks good. Equal volumes of insert and vector will be fine. [DNA] calculations are based on 8.5μL each. |
Revision as of 06:57, 28 July 2011
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ObjectiveTry to get the ligation and or transformation to work for the BSA-intein constructs. Bench work
ResultsTransformationYesterday's plates both had of colonies. After some poking around, we realized that the Ampicillin stocks made on 6/20 are only 10 mg/mL not 100 mg/mL. The plates poured yesterday were the first batch using the new Amp and are thus LBAmp10 not LBAmp100. I will be re-plating another 100μL on new LBAmp100 plates today. Gel purification |
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