User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/07/07

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AU CHEM-570 Lab Prep <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  • Try electroporation
  • Re-ligate with different I:V

Bench work

  1. Electrocompetent DH10B
    1. t=0 OD600=0.08
    2. t~1.5h OD600~0.2
    3. t~2h OD600~0.6 → stop growth
    • incubate culture ~10' on ice w/ swirling


Results

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