User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/07/08: Difference between revisions
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==Objective== | ==Objective== | ||
Continue with ligation attempts | |||
==Bench work== | ==Bench work== | ||
# | # [[User:Daniel Catt]] ran an analytical gel of ligation reactions | ||
# Re-plate electroporated DH10B | |||
#* [[User:Daniel Catt]] plated remainder of all transformations from [[../07| yesterday]] | |||
==Results== | ==Results== | ||
* | ===Transformations=== | ||
* 3 colonies on 400μL plate of NovaBlue transformed with V(His)+I | |||
* 3 colonies on 100μL plate of electroporated DH10B with V(His)+I | |||
** 1 colony on negative control (V(His) electroporated into DH10B) | |||
===Gel=== | |||
no bands visible for ligation reactions | |||
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Revision as of 14:51, 11 July 2011
 AU CHEM-570 Lab Prep
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ObjectiveContinue with ligation attempts Bench work
ResultsTransformations
Gelno bands visible for ligation reactions | |
