User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/04: Difference between revisions
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#* rest blank | #* rest blank | ||
# Based on an inability to load the digested, cleaned samples, these were re-cleaned with more extensive drying steps in order to remove supposed excess alcohol. The alcohol (2-propanol) would keep the gel load samples from resting in lanes and also inhibit ligase activity. | # Based on an inability to load the digested, cleaned samples, these were re-cleaned with more extensive drying steps in order to remove supposed excess alcohol. The alcohol (2-propanol) would keep the gel load samples from resting in lanes and also inhibit ligase activity. | ||
# Re-ligate the test ligation reactions done [../03 | yesterday]] with re-cleaned plasmids. | # Re-ligate the test ligation reactions done [[../03 | yesterday]] with re-cleaned plasmids. | ||
# 'Overloaded' ligation of pTXB1.His and pTXB1 [[../../07/27 | from 7/27]] (50μL total volume) | # 'Overloaded' ligation of pTXB1.His and pTXB1 [[../../07/27 | from 7/27]] (50μL total volume) | ||
#* 20μL ddBSA insert + 10μL pTXB1 + 5μL ligase buffer ('New') + 13μL dH<sub>2</sub>O + 2μL T4 DNA ligase | #* 20μL ddBSA insert + 10μL pTXB1 + 5μL ligase buffer ('New') + 13μL dH<sub>2</sub>O + 2μL T4 DNA ligase |
Revision as of 07:28, 9 August 2011
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Objective
Bench work
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