User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/16: Difference between revisions
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==Objective== | ==Objective== | ||
* Run QuikChange on pMXB10 from [[yesterday]](by Dr. Muratore) on gel | |||
* Check transformations on plates | |||
* Prepare for expression of pMAL-pIII and pMXB10/start cultures from colonies on plates | |||
==Bench work== | ==Bench work== | ||
# | # Ran 1.0% agarose DNA gel with [[Muratore:Protocols/PCR/QuikChange | 2-stage QuikChange]] of pMXB10 for +His tag from [[yesterday]](by Dr. Muratore) | ||
#* Lane 1: Ladder | |||
#* Lane 2: ---- | |||
#* Lane 3: ---- | |||
#* Lane 4: pMXB10 QC +His [[../15 | 8/15]] | |||
#* Lane 5: ---- | |||
#* Lane 6: ---- | |||
#* Lane 7: pMXB10 QC Ctrl [[../15 | 8/15]] | |||
==Results== | ==Results== | ||
Revision as of 14:11, 16 August 2011
 AU CHEM-570 Lab Prep
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Objective
Bench work
Results
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