User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/19: Difference between revisions
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Run DNA gel with test QuikChange from yesterday | Run DNA gel with test QuikChange from yesterday | ||
Prepare media and buffers for re-growth of cells | Prepare media and buffers for re-growth of cells | ||
QC with pMXB10 and more cycles | |||
==Bench work== | ==Bench work== | ||
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#* Lane 7: pTXB1 QC +His Stage 1, Forward 10cycles [[../18 | 8/18]], 5μL sample + 1μL loading buffer | #* Lane 7: pTXB1 QC +His Stage 1, Forward 10cycles [[../18 | 8/18]], 5μL sample + 1μL loading buffer | ||
#* Lane 10: pTXB1 QC +His Stage 1, Reverse 10cycles [[../18 | 8/18]], 5μL sample + 1μL loading buffer | #* Lane 10: pTXB1 QC +His Stage 1, Reverse 10cycles [[../18 | 8/18]], 5μL sample + 1μL loading buffer | ||
# [[Muratore:Protocols/PCR/QuikChange | 2-stage QuikChange protocol]] with pMXB10 | |||
#* Used most recent dilution to 10ng/μL, 8/15/11 | |||
#* 12 cycles in stage 1, 18 cycles in stage 2, 8 minute extension step | |||
==Results== | ==Results== | ||
Revision as of 10:53, 22 August 2011
 AU CHEM-570 Lab Prep
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ObjectiveRun DNA gel with test QuikChange from yesterday Prepare media and buffers for re-growth of cells QC with pMXB10 and more cycles Bench work
Results
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