User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/23: Difference between revisions
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#** pMXB10 #2 OD = 0.639 | #** pMXB10 #2 OD = 0.639 | ||
#* Induced expression with 500μL 1M IPTG, grew O/N @ 37°C | #* Induced expression with 500μL 1M IPTG, grew O/N @ 37°C | ||
# Followed standard [[Muratore:Protocols/Transformation | Transformation protocol]] with pMXB10 QuikChange product from [[..19 | Friday]] | # Followed standard [[Muratore:Protocols/Transformation | Transformation protocol]] with pMXB10 QuikChange product from [[../19 | Friday]] | ||
# 3' SapI primer was incorrect, so [[Muratore:Protocols/PCR/Basic | PCR]] was redone today with correct primer just come in. | # 3' SapI primer was incorrect, so [[Muratore:Protocols/PCR/Basic | PCR]] was redone today with correct primer just come in. | ||
Revision as of 13:40, 26 August 2011
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Objective
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