User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/24

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Objective

Repeat BSA cloning into intein vectors with new, correct 3' primer.

Bench work

  1. PCR purification
  • Followed instructions for Promega Gel and PCR purification kit, using spin column method
  • Purified experimental BSA PCR sample from yesterday
  1. Double-digest
Tube sterile H2O 10X NEB2 100X BSA DNA NheI (10 kU/mL) SapI (2 kU/mL
pTXB1His 14.5 μL 5 μL 0.5 μL 25 μL of 102 μg/mL 2.5μL 2.5μL
pTXB1 14.5 μL 5 μL 0.5 μL 25 μL of 120 μg/mL 2.5μL 2.5μL
BSA PCR 0 μL 5 μL 0.5 μL 39.5 μL from purified BSA PCR reaction in step 1 2.5μL 2.5μL
  2. Gel purification
    • Kathryn Muratore 17:28, 24 August 2011 (EDT):I forgot to phosphatase the vectors before loading the gel, so we will phosphatase after purification.
    1. 50μL pTXB1His double-digest from step 2 + 10μL 6X loading buffer
    2. 10μL 1KB ladder
    3. skip
    4. 50μL pTXB1 double-digest from step 2 + 10μL 6X loading buffer
    5. 50μL BSA PCR double-digest from step 2 + 10μL 6X loading buffer

Results

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