User:Katpak

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== Lab Experience==
== Lab Experience==
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Summer 2007: I worked at Caltech on the iGEM project. Specifically, I was working on the design and implementation of riboregulators.
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'''MIT Department of Biological Engineering '''
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January 2006-June 2007: I worked in Prof. Shuguang Zhang's lab at MIT on a fusion protein for error correction in DNA synthesis.
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August 2004-August 2005: I worked at the Public Health Research Institute in Newark, New Jersey on developing a diagnostic assay to detect pathogenic species in humans with molecular beacons.
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Cambridge, MA
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Faculty Advisor: Bevin Engelward, direct advisor Dr. Werner Olipitz
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Examined the effects of radiation on homologous recombination in mouse pancreata. Working with the fluorescent yellow direct repeat mouse model, I used in situ imaging and flow cytometry to determine the level of induced HR in mouse pancreata as a result of exposure to varying levels of radiation. Publication is pending for the upcoming year on this work. I also recently began work on measuring T cell activation and response to radiation. I will be involved with quantifying cytokine levels in irradiated mice as well as histological comparisons of the mouse gastrointestinal system. I was also responsible for upkeep of the mouse colony and assisted on several other projects in the lab. (January 2008-Present)
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* [[Katpak:m1d1| 20.109 Mod1Day1 Q&A]]
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* [[Katpak:m1d2| 20.109 Mod1Day2 Q&A]]
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* [[Katpak:m3d2| 20.109 Mod3 Research Proposal]]
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'''California Institute of Technology '''
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Pasadena, CA
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Faculty Advisor: Christina Smolke
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Designed and characterized regulatory RNA elements for controlled gene expression. This work was part of a larger project which was entered into the International Genetically Engineered Machines Competition at MIT (iGEM) in fall of 2007. The goal of the project was to regulate the lysis/lysogeny switch in lambda phage upon infection of E. coli. With the help of RNAstructure and VectorNTI software, I designed thirteen interacting RNA elements that would control expression of life cycle genes in lambda phage and measured their interaction with flow cytometry (http://openwetware.org/wiki/IGEM:Caltech/2007/Project/Riboregulator). My research was funded by the Amgen Scholars program. (June 2007-August 2007)
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'''MIT Media Lab '''
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Cambridge, MA
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Faculty Advisor: Dr. Peter Carr
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Assisted in testing proteins for error correction in DNA synthesis. The goal of the project was to make DNA synthesis faster and more efficient. I was responsible for expression of an engineered protein which would detect and correct errors in DNA synthesis. The bulk of my work involved protein expression and solubilization. (January 2006-June 2007)
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'''Public Health Research Institute'''
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Newark, NJ
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Advisor: Dr. Dan-Oscar Antson
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Assisted in developing a diagnostic assay to detect pathogenic species in humans using fluorescent hybridization probes called molecular beacons. My responsibilities were to quantify levels of beacon-target interactions using real-time PCR. (August 2004-August 2005)

Revision as of 02:52, 20 November 2008


Ekaterina (Kat) Pak

Department of Biological Engineering
MIT
Cambridge, MA

katpak@mit.edu



Education

Biological Engineering
MIT, Class of 2009


Lab Experience

MIT Department of Biological Engineering

Cambridge, MA

Faculty Advisor: Bevin Engelward, direct advisor Dr. Werner Olipitz

Examined the effects of radiation on homologous recombination in mouse pancreata. Working with the fluorescent yellow direct repeat mouse model, I used in situ imaging and flow cytometry to determine the level of induced HR in mouse pancreata as a result of exposure to varying levels of radiation. Publication is pending for the upcoming year on this work. I also recently began work on measuring T cell activation and response to radiation. I will be involved with quantifying cytokine levels in irradiated mice as well as histological comparisons of the mouse gastrointestinal system. I was also responsible for upkeep of the mouse colony and assisted on several other projects in the lab. (January 2008-Present)


California Institute of Technology

Pasadena, CA

Faculty Advisor: Christina Smolke

Designed and characterized regulatory RNA elements for controlled gene expression. This work was part of a larger project which was entered into the International Genetically Engineered Machines Competition at MIT (iGEM) in fall of 2007. The goal of the project was to regulate the lysis/lysogeny switch in lambda phage upon infection of E. coli. With the help of RNAstructure and VectorNTI software, I designed thirteen interacting RNA elements that would control expression of life cycle genes in lambda phage and measured their interaction with flow cytometry (http://openwetware.org/wiki/IGEM:Caltech/2007/Project/Riboregulator). My research was funded by the Amgen Scholars program. (June 2007-August 2007)


MIT Media Lab

Cambridge, MA

Faculty Advisor: Dr. Peter Carr

Assisted in testing proteins for error correction in DNA synthesis. The goal of the project was to make DNA synthesis faster and more efficient. I was responsible for expression of an engineered protein which would detect and correct errors in DNA synthesis. The bulk of my work involved protein expression and solubilization. (January 2006-June 2007)


Public Health Research Institute

Newark, NJ

Advisor: Dr. Dan-Oscar Antson

Assisted in developing a diagnostic assay to detect pathogenic species in humans using fluorescent hybridization probes called molecular beacons. My responsibilities were to quantify levels of beacon-target interactions using real-time PCR. (August 2004-August 2005)

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