User:Kchang17/notes: Difference between revisions
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m (→June 29, 2006) |
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*Q04740 | *Q04740 | ||
*I14103 | *I14103 | ||
*I14104 | *I14104 - having trouble cloning this, have to wait on testing | ||
*Q04720 | *Q04720 | ||
*Q04730 | *Q04730 | ||
**during construction, a point mutation occurred in the ORF of the repressor protein (C0073) resulting in a change from Phe to Leu | **during construction, a point mutation occurred in the ORF of the repressor protein (C0073) resulting in a change from Phe to Leu | ||
Revision as of 09:40, 27 June 2006
June 22, 2006
- tested everything in SP1.0 at 6 arabinose levels (0%-10-4%)
- Q04400.007 innoculated at OD 0.0005 and grew for ~10 hrs (picture)
- Q04740 exptl cultures grew too dense - performed an extra dilution and redid the innoculation - innoculated at OD 0.0005 and grew for ~14 hrs
- I14103 - grew very slowly - innoculated at OD 0.001 and grew for ~14 hrs (grew a little too dense)
June 29, 2006
- Q04740
- I14103
- I14104 - having trouble cloning this, have to wait on testing
- Q04720
- Q04730
- during construction, a point mutation occurred in the ORF of the repressor protein (C0073) resulting in a change from Phe to Leu
