User:Kchang17/notes: Difference between revisions
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==March 15, 2006== | ==March 15, 2006== | ||
*first characterization of mutated tetR QPI (obtained from inverter library) ([http://openwetware.org/wiki/Image:March15_2006_Q04400.007_MATLAB.png | *first characterization of mutated tetR QPI (obtained from inverter library) ([http://openwetware.org/wiki/Image:March15_2006_Q04400.007_MATLAB.png MATLAB analysis]) | ||
==June 22, 2006== | ==June 22, 2006== |
Revision as of 14:36, 10 July 2006
February 29, 2006
- characterization of penI QPI in I13537.pSB1A3 with three replicates (MATLAB analysis)
March 15, 2006
- first characterization of mutated tetR QPI (obtained from inverter library) (MATLAB analysis)
June 22, 2006
- tested everything in SP1.0 at 6 arabinose levels (0%-10-4%)
- Q04400.007 innoculated at OD 0.0005 and grew for ~10 hrs (picture) (MATLAB analysis)
- Q04740 exptl cultures grew too dense - performed an extra dilution and redid the innoculation - innoculated at OD 0.0005 and grew for ~14 hrs
- I14103 - grew very slowly - innoculated at OD 0.001 and grew for ~14 hrs (grew a little too dense)
June 29, 2006
- devices in SP1.0
- Q04720 - innoculated at 0.0003 - grew ~15 hrs (too dense) (picture)
- Q04730 - innoculated at 0.0003 - grew ~16 hrs (too dense) (picture)
- during construction, a point mutation occurred in the ORF of the repressor protein (C0073) resulting in a change from Phe to Leu
- Q04740 - innoculated 0.0003 - grew ~16 hrs (too dense)
- I14103 - innoculated at 0.0005 - grew ~15 hrs (too dense)
- I14104 - having trouble cloning this, have to wait on testing
July 11, 2006
- grew to stationary, then diluted back 100x, grew for ~8 hrs, innoculated at ~11:30 p.m.
- devices in SP1.0
- Reshma's constructs:
- B0030.C2002.B0015.p8 (A) - innoculated at 0.0001 - grew ~14.5 hrs
- B0030.C2003.B0015.p8 (B) - innoculated at 0.0001
- Q04740 red colony (C) (from streaked plate of FACS run) - innoculated at 0.0001 - grew ~14.5 hrs
- Q04740 white (pink) colony (D) (from streaked plate of FACS run) - innoculated at 0.0001 - grew ~14.5 hrs
- empty SP1.0 (E) - innoculated at 0.0001 - grew ~14.5 hrs
- Q04400.007 (F) - innoculated at 0.0001 - grew ~14.5 hrs