User:Kchang17/notes: Difference between revisions
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*tested everything in SP1.0 at 6 arabinose levels (0%-10<sup>-4</sup>%) | *tested everything in SP1.0 at 6 arabinose levels (0%-10<sup>-4</sup>%) | ||
*Q04400.007 innoculated at OD 0.0005 and grew for ~10 hrs ([http://openwetware.org/wiki/Image:June22_2006-Q04400mut-rep1.png picture]) ([http://openwetware.org/wiki/Image:June22_2006_tetR_QPI_rep1.png MATLAB analysis]) | *Q04400.007 innoculated at OD 0.0005 and grew for ~10 hrs ([http://openwetware.org/wiki/Image:June22_2006-Q04400mut-rep1.png picture]) ([http://openwetware.org/wiki/Image:June22_2006_tetR_QPI_rep1.png MATLAB analysis]) | ||
**note: 10 hrs probably wasn't enough time for it to reach steady state esp. at high induction, assuming inverter was working better on [http://openwetware.org/wiki/Image:July11_2006_tetR.png July 11] | |||
*Q04740 exptl cultures grew too dense - performed an extra dilution and redid the innoculation - innoculated at OD 0.0005 and grew for ~14 hrs | *Q04740 exptl cultures grew too dense - performed an extra dilution and redid the innoculation - innoculated at OD 0.0005 and grew for ~14 hrs | ||
*I14103 - grew very slowly - innoculated at OD 0.001 and grew for ~14 hrs (grew a little too dense) | *I14103 - grew very slowly - innoculated at OD 0.001 and grew for ~14 hrs (grew a little too dense) |
Revision as of 12:06, 11 July 2006
February 29, 2006
- characterization of penI QPI in I13537.pSB1A3 with three replicates (MATLAB analysis)
March 15, 2006
- first measurement of mutated tetR QPI (obtained from inverter library) in SP1.0 (MATLAB analysis)
June 22, 2006
- tested everything in SP1.0 at 6 arabinose levels (0%-10-4%)
- Q04400.007 innoculated at OD 0.0005 and grew for ~10 hrs (picture) (MATLAB analysis)
- note: 10 hrs probably wasn't enough time for it to reach steady state esp. at high induction, assuming inverter was working better on July 11
- Q04740 exptl cultures grew too dense - performed an extra dilution and redid the innoculation - innoculated at OD 0.0005 and grew for ~14 hrs
- I14103 - grew very slowly - innoculated at OD 0.001 and grew for ~14 hrs (grew a little too dense)
June 29, 2006
- devices in SP1.0, six arabinose levels (0%-10-4%)
- Q04720 - innoculated at 0.0003 - grew ~15 hrs (too dense) (picture)
- Q04730 - innoculated at 0.0003 - grew ~16 hrs (too dense) (picture)
- during construction, a point mutation occurred in the ORF of the repressor protein (C0073) resulting in a change from Phe to Leu
- Q04740 - innoculated 0.0003 - grew ~16 hrs (too dense) - weirdness, where did GFP and RFP go?
- I14103 - innoculated at 0.0005 - grew ~15 hrs (too dense) (picture)
- I14104 - having trouble cloning this, have to wait on testing
July 6, 2006
- induced ~7 hrs at no and high induction
- the single red colony on a plate from Q04740 in SP1.0 glycerol - got high RFP signal and GFP tracked induction - streaked this culture for July 11 testing
- one white colony from the same plate - GFP showed this time - background RFP
- empty SP1.0 - showed GFP and RFP
July 11, 2006
- grew to stationary, then diluted back 100x, grew for ~8 hrs, innoculated at ~11:30 p.m.
- devices in SP1.0, six arabinose levels (0%-10-4%)
- Reshma's constructs:
- Q04740 red colony (C) (from streaked plate of FACS run) - innoculated at 0.0001 - grew ~14.5 hrs [picture]
- Q04740 white (pink) colony (D) (from streaked plate of FACS run) - innoculated at 0.0001 - grew ~14.5 hrs [picture]
- empty SP1.0 (E) - innoculated at 0.0001 - grew ~14.5 hrs [picture]
- Q04400.007 (F) - innoculated at 0.0001 - grew ~14.5 hrs [picture]