User:Kchang17/notes

June 22, 2006

• tested everything in SP1.0 at 6 arabinose levels (0%-10^-4%)
• Q04400.007 innoculated at OD 0.0005 and grew for ~10 hrs (picture) (MATLAB analysis)
• Q04740 exptl cultures grew too dense - performed an extra dilution and redid the innoculation - innoculated at OD 0.0005 and grew for ~14 hrs
• I14103 - grew very slowly - innoculated at OD 0.001 and grew for ~14 hrs (grew a little too dense)

June 29, 2006

• devices in SP1.0
• Q04720 - innoculated at 0.0003 - grew ~15 hrs (too long!)
• Q04730 - innoculated at 0.0003 - grew ~16 hrs (too long!)
  • during construction, a point mutation occurred in the ORF of the repressor protein (C0073) resulting in a change from Phe to Leu
• Q04740 - innoculated 0.0003 - grew ~16 hrs (too long!)
• I14103 - innoculated at 0.0005 - grew ~15 hrs (too long!)
• I14104 - having trouble cloning this, have to wait on testing

July 11, 2006

• grew to stationary, then diluted back 100x, grew for ~8 hrs, innoculated at ~11 p.m.
• devices in SP1.0
• Reshma's constructs:
  • B0030.C2002.B0015.p8 (A) - innoculated at 0.0001
  • B0030.C2003.B0015.p8 (B) - innoculated at 0.0001
• Q04740 red colony (C) (from streaked plate of FACS run) - innoculated at 0.0001
• Q04740 white (pink) colony (D) (from streaked plate of FACS run) - innoculated at 0.0001
• empty SP1.0 (E) - innoculated at 0.0001
• Q04400.007 (F) - innoculated at 0.0001